Simultaneous Activation of SHR and ATHB8 Expression Defines Switch to Preprocambial Cell State in Arabidopsis Leaf Development
Simultaneous Activation of SHR and ATHB8 Expression Defines Switch to Preprocambial Cell State in Arabidopsis Leaf Development
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DOI：
10.1002/dvdy.22516
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发表时间：
2011-01-01
影响因子：
2.5
通讯作者：
Scarpella, Enrico
中科院分区：
文献类型：
--
作者：
Gardiner, Jason;Donner, Tyler J.;Scarpella, Enrico
叶维管网络形成的过程长期以来一直引起发育生物学家的关注，特别是因为细长的维管前体原形成层细胞的文件似乎与明显等效的等径地面细胞不同。在拟南芥叶子中，被指定为维管命运的地面细胞参与拟南芥 HOMEOBOX8 (ATHB8) 的表达。虽然表达 ATHB8 的地面细胞的转录状态的定义特别有用，但尚未鉴定出在此阶段启动表达的其他基因。在这里，我们发现在叶子发育过程中，SHORT-ROOT (SHR) 的表达与 ATHB8 的表达同时被激活。在操纵静脉模式的条件下，SHR 和 ATHB8 表达域之间的一致性仍然存在，这表明 SHR 和 ATHB8 表达的开始确定了向预示静脉形成的前前室细胞状态的转变。我们的观察进一步表征了叶脉发育的解剖学上不明显阶段的细胞的分子特性。发展动态 240: 261-270, 2011。(C) 2010 Wiley-Liss, Inc.
The processes underlying the formation of leaf vascular networks have long captured the attention of developmental biologists, especially because files of elongated vascular-precursor procambial cells seem to differentiate from apparently equivalent, isodiametric ground cells. In Arabidopsis leaves, ground cells that have been specified to vascular fate engage expression of ARABIDOPSIS THALIANA HOMEOBOX8 (ATHB8). While definition of the transcriptional state of ATHB8-expressing ground cells would be particularly informative, no other genes have been identified whose expression is initiated at this stage. Here we show that expression of SHORT-ROOT (SHR) is activated simultaneously with that of ATHB8 in leaf development. Congruence between SHR and ATHB8 expression domains persists under conditions of manipulated vein patterning, suggesting that inception of expression of SHR and ATHB8 identifies transition to a preprocambial cell state that presages vein formation. Our observations further characterize the molecular identity of cells at anatomically inconspicuous stages of leaf vein development. Developmental Dynamics 240: 261-270, 2011. (C) 2010 Wiley-Liss, Inc.