Nitrogen removal characteristics and predicted conversion pathways of a heterotrophic nitrification–aerobic denitrification bacterium, Pseudomonas aeruginosa P-1
Nitrogen removal characteristics and predicted conversion pathways of a heterotrophic nitrification–aerobic denitrification bacterium, Pseudomonas aeruginosa P-1
复制标题
异养硝化好氧反硝化细菌铜绿假单胞菌 P-1 的脱氮特性及预测转化途径
DOI：
10.1007/s11356-020-11066-7
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发表时间：
2021
影响因子：
5.8
通讯作者：
Linna Du
中科院分区：
文献类型：
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作者：
Ran Wei;Cai Hui;Yiping Zhang;Hui Jiang;Yuhua Zhao;Linna Du
本文研究了铜绿假单胞菌（P-1）的异养硝化-好氧反硝化活性，揭示了其氮素转化途径。该菌株对NH 4+、NO3-和NO2-的最高去除率（分别为9.29、6.12和3.72 mg L−1h−1）高于大多数已报道的细菌，并且在碳源为葡萄糖、C/N比为15、pH为8、温度为30 °C、振荡速度为200 rpm时达到。首次研究了铜绿假单胞菌对3种氮源的去除顺序和特性。结果表明，P-1优先硝化NH 4+，只有当NH 4+几乎完全耗尽时才开始硝化NO2-和NO3-。氮源的同位素标记显示，P-1使用部分和完全硝化/反硝化途径，可以同时或独立运行，这取决于不同类型的氮化合物的可用性，N2作为最终的气态产物，几乎没有NO2−积累。此外，P-1菌株可以在高盐度（40 g L−1）和高浓度的Cu 2+、Zn 2+、Cr 6+、Pb 2+和Cd 2+（50 mg L−1）下转化各种氮化合物。因此，P-1可作为无机脱氮菌的替代菌株应用于实际生产中。
In this study, the heterotrophic nitrification–aerobic denitrification activity ofPseudomonas aeruginosa(P-1) strain was investigated, and the N transformation pathway was revealed. The highest removal rates of NH4+, NO3−, and NO2−(9.29, 6.12, and 3.72 mg L−1h−1, respectively) by this strain were higher than those by most reported bacteria and were achieved when the carbon source was glucose, C/N ratio was 15, pH was 8, temperature was 30 °C, and shaking speed was 200 rpm. The removal order and characteristics of three N sources were investigated inPseudomonas aeruginosafor the first time. The results revealed that P-1 preferentially nitrified NH4+and only began to denitrify NO2−and NO3−when NH4+was almost entirely depleted. Isotopic labeling of N sources revealed that P-1 uses both partial and complete nitrification/denitrification pathways that can operate either simultaneously or independently, depending on the availability of different types of N compounds, with N2as the final gaseous product and virtually no NO2−accumulation. Moreover, the P-1 strain could convert various nitrogen compounds under high salinity (40 g L−1) and high concentrations of Cu2+, Zn2+, Cr6+, Pb2+, and Cd2+(50 mg L−1). Therefore, P-1 could be used as an alternative of inorganic N-removal bacteria in practical applications.