A zinc selective oxytocin based biosensor
A zinc selective oxytocin based biosensor
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DOI：
10.1039/c9tb01932d
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发表时间：
2020-01-07
影响因子：
7
通讯作者：
Yitzchaik, Shlomo
中科院分区：
文献类型：
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作者：
Mervinetsky, Evgeniy;Alshanski, Israel;Yitzchaik, Shlomo
与其他金属离子相比，催产素是一种肽激素，对 Zn2+ 和 Cu2+ 离子具有高亲和力。这种亲和力使催产素成为监测生物液中这些必需离子水平的有吸引力的识别层。天然催产素无法区分 Cu2+ 和 Zn2+ 离子，因此在存在 Cu2+ 的情况下它无法用于感测 Zn2+。我们通过理论计算阐明了催产素末端胺基对 Cu2+ 亲和力的影响。我们设计了一种新型 Zn2+ 选择性催产素生物传感器，利用末端胺进行表面锚定，同时防止对 Cu2+ 的响应。该生物传感器在阻抗生物传感中对 Zn2+ 显示出卓越的选择性和非常高的灵敏度。这项研究首次展示了一种催产素衍生传感器，可直接用于在存在 Cu2+ 的情况下感测 Zn2+。
Oxytocin is a peptide hormone with high affinity to both Zn2+ and Cu2+ ions compared to other metal ions. This affinity makes oxytocin an attractive recognition layer for monitoring the levels of these essential ions in biofluids. Native oxytocin cannot differentiate between Cu2+ and Zn2+ ions and hence it is not useful for sensing Zn2+ in the presence of Cu2+. We elucidated the effect of the terminal amine group of oxytocin on the affinity toward Cu2+ using theoretical calculations. We designed a new Zn2+ selective oxytocin-based biosensor that utilizes the terminal amine for surface anchoring, also preventing the response to Cu2+. The biosensor shows exceptional selectivity and very high sensitivity to Zn2+ in impedimetric biosensing. This study shows for the first time an oxytocin derived sensor that can be used directly for sensing Zn2+ in the presence of Cu2+.