Simultaneous Intake of Euglena gracilis and Vegetables Exerts Synergistic Anti-Obesity and Anti-Inflammatory Effects by Modulating the Gut Microbiota in Diet-Induced Obese Mice
Simultaneous Intake of Euglena gracilis and Vegetables Exerts Synergistic Anti-Obesity and Anti-Inflammatory Effects by Modulating the Gut Microbiota in Diet-Induced Obese Mice
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DOI：
10.3390/nu11010204
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发表时间：
2019-01-01
期刊：
影响因子：
5.9
通讯作者：
Tsuduki, Tsuyoshi
中科院分区：
文献类型：
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作者：
Okouchi, Ran;Shuang, E.;Tsuduki, Tsuyoshi
我们确定了是否由食用富含不溶性膳食纤维的纤细裸藻（Euglena gracilis）提供的抗肥胖效果可以通过共同食用富含可溶性膳食纤维的蔬菜来增强。将9周龄雄性C57 BL/6 J小鼠分为5组：第1组接受正常饮食，第2组接受高脂饮食，第3、4和5组分别接受含有0.3%副淀粉、1.0%裸藻或1.0%裸藻加0.3%蔬菜（大麦叶、羽衣甘蓝和明日叶）的高脂饮食。小鼠自由采食直至18周龄。裸藻的摄入量显着减少了肥胖小鼠的内脏脂肪积累，和蔬菜的共同消费增强了这一效果。与蔬菜一起食用裸藻减少了脂肪细胞面积，抑制了与脂肪酸合成相关的基因的表达，上调了与脂肪细胞脂解相关的基因，并抑制了炎症的血清标志物。值得注意的是，我们还观察到产生短链脂肪酸的有益细菌的比例增加，导致炎症的有害细菌减少，以及短链脂肪酸产量增加。因此，同时食用蔬菜可能通过调节肠道微生物群组成来增强眼虫的抗肥胖和抗炎作用。
We determined whether the anti-obesity effect provided by the consumption of Euglena gracilis (Euglena), which is rich in insoluble dietary fiber, could be enhanced by the co-consumption of vegetables with an abundance of soluble dietary fiber. Nine-week-old male C57BL/6J mice were divided into five groups as follows: group 1 received a normal diet, group 2 received a high-fat diet, and groups 3, 4, and 5 received high fat diets containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale, and ashitaba), respectively. Mice were fed ad libitum until 18 weeks of age. Euglena intake significantly reduced visceral fat accumulation in obese mice, and co-consumption of vegetables enhanced this effect. Consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis, upregulated genes related to adipocyte lipolysis, and suppressed serum markers of inflammation. Notably, we also observed an increase in the fraction of short-chain fatty acid-producing beneficial bacteria, a reduction in harmful bacteria that cause inflammation, and an increase in short-chain fatty acid production. Therefore, the co-consumption of vegetables enhanced the anti-obesity and anti-inflammatory effects of Euglena, likely by modulating the gut microbiota composition.