Disclosing hidden transcripts: Mouse natural sense-antisense transcripts tend to be poly(A) negative and nuclear localized
Disclosing hidden transcripts: Mouse natural sense-antisense transcripts tend to be poly(A) negative and nuclear localized
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DOI：
10.1101/gr.3155905
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发表时间：
2005-04-01
期刊：
影响因子：
7
通讯作者：
Abe, K
中科院分区：
文献类型：
--
作者：
Kiyosawa, H;Mise, N;Abe, K
全基因组计算机分析在小鼠转录组中鉴定了数千个天然的有义-反义转录物（SAT）对。我们使用链特异性寡核苷酸微阵列研究了它们的表达，该微阵列从1947个SAT对中区分正义和反义RNA的表达。大多数预测的SAT在各种组织中以各种稳态水平表达，并且阵列数据的聚类分析表明，一些SAT的正义和反义表达的比率在这些组织中显著波动，而其余的则没有变化。令人惊讶的是，进一步的分析表明，大量的多重大小的转录本从SAT基因座表达，这往往是多聚（A）阴性，和核定位。SAT通常不被多聚腺苷酸化的趋势是保守的，即使在植物拟南芥中随机选择的SAT基因中也是如此。这些共同的特征意味着SAT在基因表达调控中的一般作用。
Genome-wide in silico analysis identified thousands of natural sense-antisense transcript (SAT) pairs in the mouse transcriptome. We investigated their expression using strand-specific oligo-microarray that distinguishes expression of sense and antisense RNA from 1947 SAT pairs. The majority of the predicted SATs are expressed at various steady-state levels in various tissues, and cluster analysis of the array data demonstrated that the ratio of sense and antisense expression for some of the SATs fluctuated markedly among these tissues, while the rest was unchanged. Surprisingly, further analyses indicated that vast amounts of multiple-sized transcripts are expressed from the SAT loci, which tended to be poly(A) negative, and nuclear localized. The tendency that the SATs are often not polyadenylated is conserved, even in the randomly chosen SAT genes in the plant Arabidopsis thaliana. Such common characteristics imply general roles of the SATs in regulation of gene expression.