[Relationship between Myofibril Fragmentation Index and Postmortem Interval].
[Relationship between Myofibril Fragmentation Index and Postmortem Interval].
复制标题
[肌原纤维破碎指数与死后间隔的关系]。
DOI：
10.3969/j.issn.1004-5619.2017.06.004
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Z. Zhao
中科院分区：
文献类型：
--
作者：
L. Shen;R. Z. Rui;J. Bian;M. He;Z. Zhao
目标
探讨人骨骼肌肌原纤维断裂指数（MFI）与死亡时间（PMI）的关系。
方法
采用双缩脲法在室温下测定不同PMI下人右肱二头肌和右股四头肌的蛋白质含量。用紫外分光光度计测定骨骼肌在540 nm处的MFI。以死亡时间为自变量（x），MFI为因变量（y）进行回归分析。
结果
PMI早期，随着PMI的延长，人体骨骼肌的MFI明显增加，到12 h达到峰值，然后趋于稳定。死亡后12h内，右侧肱二头肌和右侧股四头肌的回归方程分别为y=32.660+ 3.227x（r= 0.9879）和y=32.380+ 3.495x（r= 0.9839）。
结论
MFI与PMI之间存在高度的相关性。结合法医学实践，MFI可用于PMI早期（尤其是12 h）的判断。
OBJECTIVES
To study the relationship between myofibril fragmentation index （MFI） of human skeletal muscle and postmortem interval （PMI）.
METHODS
The protein concentrations of human right biceps brachii muscle and right quadriceps femoris muscle were obtained at different PMI, and detected at room temperature by biuret method. The MFI of skeletal muscle at 540 nm was measured by ultraviolet spectrophotometer. Regression analysis was performed with time of death as independent variable （x） and MFI as dependent variable （y）.
RESULTS
In early PMI, the MFI of human skeletal muscle increased obviously according to the prolongation of PMI, and peaking by 12 h and then tended to steady. Within 12 h after death, the regression equations of right biceps brachii muscle and right quadriceps femoris muscle were y=32.660+3.227 x（r=0.987 9） and y=32.380+3.495 x（r=0.983 9）, respectively.
CONCLUSIONS
There's high correlation between MFI and PMI. Combining with forensic practice, MFI can be used for the estimation of early PMI （especially in 12 h）.