Dysregulated Interleukin 11 in primary Sjögren's Syndrome contributes to apoptosis of glandular epithelial cells
Dysregulated Interleukin 11 in primary Sjögren's Syndrome contributes to apoptosis of glandular epithelial cells
复制标题
原发性干燥综合征中白细胞介素 11 失调导致腺上皮细胞凋亡
DOI：
10.1002/cbin.11236
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发表时间：
2020
影响因子：
3.9
通讯作者：
Lingyan Zheng
中科院分区：
文献类型：
--
作者：
Lei Ye;Huan Shi;Shufeng Wu;Chuangqi Yu;Baoli Wang;Lingyan Zheng
本研究旨在探讨白细胞介素-11（IL-11）在原发性干燥综合征（pSS）发病机制中的潜在作用。采用真实的实时聚合酶链反应检测30例pSS患者和30例健康对照者唇腺中IL-11的表达。进行免疫组织化学以评估IL-II-阳性细胞在唇腺中的分布。使用人唾液腺（HSG）细胞系研究IL-11对体外腺上皮细胞的作用。分别通过CCK-8试剂盒和EdU测定法检测细胞活力和细胞增殖。流式细胞仪检测凋亡细胞数，Annexin V/PI和Hoechst染色。我们发现IL-11的表达水平在pSS唇腺中显著降低，并且与C反应蛋白水平呈正相关，与类风湿因子水平呈负相关。与健康对照患者相比，在pSS患者的唇腺中观察到较少数量的腺上皮细胞被IL-11抗体阳性染色。经IL-11处理后，HSG细胞的存活率和增殖能力明显高于对照组。IL-11处理后组中HSG细胞的总凋亡率和坏死率显著降低。总之，结果表明IL-11促进腺上皮细胞的活力和增殖，并抑制腺上皮细胞的凋亡和坏死率。在pSS中，下调的IL-11可能有助于唾液腺上皮细胞的凋亡。然而，它可能是一个潜在的目标，以减轻病理性萎缩的腺上皮细胞在pSS患者。
The purpose of this study was to explore the potential function of interleukin‐11 (IL‐11) in the pathogenesis of primary Sjögren's syndrome (pSS) patients. Real‐time polymerase chain reaction was performed to examine IL‐11 expression in the labial glands of 30 pSS patients and 30 healthy controls. Immunohistochemistry was conducted to assess the distribution of IL‐ll‐positive cells in labial glands. The human salivary gland (HSG) cell line was used to study the effects of IL‐11 on gland epithelial cells in vitro. Cell viability and cell proliferation were examined by CCK‐8 kit and EdU assay, respectively. The population of apoptotic cells was detected in flow cytometry followed by Annexin V/PI and Hoechst staining. We found that the expression levels of IL‐11 were remarkably decreased in pSS labial glands and were positively correlated with C‐reactive protein levels and negatively correlated with rheumatoid factor levels. Fewer numbers of glandular epithelial cells were observed to be positively stained with IL‐11 antibody in labial glands from pSS patients than those in healthy control patients. After IL‐11 treatment, the viability and proliferation of HSG cells were significantly higher than those in the control group. The total apoptotic and necrotic rates of HSG cells in the group after IL‐11 treatment were significantly lower. In conclusion, the results indicated that IL‐11 promoted viability and proliferation and inhibited apoptotic and necrotic rates of glandular epithelial cells. In pSS, downregulated IL‐11 might contribute to the apoptosis of salivary gland epithelial cells. However, it might be a potential target to alleviate the pathological atrophy of glandular epithelial cells in pSS patients.