Methylation of the FGFR2 gene is associated with high birth weight centile in humans
Methylation of the FGFR2 gene is associated with high birth weight centile in humans
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DOI：
10.2217/epi.14.40
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发表时间：
2014-01-01
期刊：
影响因子：
3.8
通讯作者：
Fryer, Anthony A.
中科院分区：
文献类型：
--
作者：
Haworth, Kim E.;Farrell, William E.;Fryer, Anthony A.
目的：本研究探讨DNA甲基化与出生体重百分位数（BWC）之间的联系，并探讨遗传变异的影响。材料和方法：使用HumanMethylation 450阵列，我们检查了BWC较低（第85百分位数）的新生儿脐带血中的候选基因相关CpG（n = 12）。在一个研究队列（n = 110）中使用焦磷酸测序和基因分型对候选人进行检查，以确定使用标准PCR进行的推定甲基化相关多态性。结果：阵列分析确定了314个与BWC极端相关的候选基因，其中4个显示= 4个BWC连锁CpG。其中，PM20 D1和MI 886表明基因决定的甲基化水平。然而，FGFR 2中三个CpG的甲基化仍然与高BWC显著相关（p = 0.004-0.027）。结论：我们发现了一个新的生物学上合理的候选人（FGFR 2）与生物武器公约，值得进一步研究。
Aims: This study examined links between DNA methylation and birth weight centile (BWC), and explored the impact of genetic variation. Materials & methods: Using HumanMethylation450 arrays, we examined candidate gene-associated CpGs in cord blood from newborns with low (85th centile) BWC (n = 12). Candidates were examined in an investigation cohort (n = 110) using pyrosequencing and genotyping for putative methylation-associated polymorphisms performed using standard PCR. Results: Array analysis identified 314 candidate genes associated with BWC extremes, four of which showed = 4 BWC-linked CpGs. Of these, PM20D1 and MI886 suggested genetically determined methylation levels. However, methylation at three CpGs in FGFR2 remained significantly associated with high BWC (p = 0.004-0.027). Conclusion: We identified a novel biologically plausible candidate (FGFR2) for with BWC that merits further study.