Comparative QTL Mapping of Resistance to Sporisorium reiliana in Maize Based on Meta-analysis of QTL Locations
Comparative QTL Mapping of Resistance to Sporisorium reiliana in Maize Based on Meta-analysis of QTL Locations
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发表时间：
2007
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通讯作者：
Hao Zhuan-fang
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作者：
Hao Zhuan-fang
以遗传图谱IBM2 2005 Neighbors为参考，利用22个玉米抗黑穗病的QTL，构建了玉米抗黑穗病的整合QTL图谱。这22个QTL分布在除第7染色体外的9条染色体上。利用meta分析方法，在2号和3号染色体上鉴定出2个“一致”QTL，图谱间隔分别为8.79cM和18.92cM。从玉米GDB网站上下载2个“共识”QTL内的基因和标记序列，利用BLASTx软件与其他白豆病抗性基因进行比对，鉴定出4个位置候选基因。采用基于玉米遗传图谱与水稻物理图谱比对的同源保守方法，将69个水稻和玉米抗性基因投射到玉米遗传图谱IBM2 2005 Neighbors上，在2个“共识QTL”中各发现1个水稻抗性基因。结果为精细制图提供了有用的信息！玉米抗黑穗病的QTL、基因克隆与分子育种。
The integration QTL map for Sporisorium reiliana resistance in maize was constructed by compiling a total of 22 QTL available for this trait with Genetic Map IBM2 2005 Neighbors as reference.These 22 QTL were found to be located on 9 chromosomes of maize with exception of chromosome 7.Using a meta-analysis method,2 "consensus" QTL were identified on chromosomes 2 and 3 with map interval of 8.79cM and 18.92cM,respectively.The gene and marker sequences within 2 "consensus" QTL were downloaded respectively from website Maize GDB,and 4 positional candidate genes were identified by comparing these sequences against resistance genes of other Poacease by internet software BLASTx.Using a synteny conservation approach based on comparative mapping between maize genetic map and rice physical map,a total of 69 rice and maize resistance genes were projected onto maize genetic map IBM2 2005 Neighbors,and one each rice resistance gene was found in 2 "consensus QTL".The results provided useful information for fine mapping!QTL,gene cloning and molecular breeding for resistance to Sporisorium reiliana in maize.