Imaging the traction stresses exerted by locomoting cells with the elastic substratum method
Imaging the traction stresses exerted by locomoting cells with the elastic substratum method
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DOI：
10.1016/s0006-3495(96)79767-9
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发表时间：
1996-04-01
影响因子：
3.4
通讯作者：
Jacobson, K
中科院分区：
文献类型：
--
作者：
Dembo, M;Oliver, T;Jacobson, K
运动细胞或贴壁细胞的细胞骨架活动经常引起足够顺应性的底物的可检测移位。根据经典的小应变平面应力弹性理论，讨论了这一现象的物理机制。这种分析的主要结果是将弹性衬底的位移场表示为牵引场上的积分。这种变换的存在被用来推导出一种贝叶斯方法，用于将基板位移的噪声测量转换为贴壁或移动的细胞施加的实际牵引力的图像。最后，讨论了统计方法的蒙特卡罗验证，介绍了一些新的薄膜流变学研究，并给出了一个实际应用。
The cytoskeletal activity of motile or adherent cells is frequently seen to induce detectable displacements of sufficiently compliant substrata. The physics of this phenomenon is discussed in terms of the classical theory of small-strain, plane-stress elasticity. The main result of such analysis is a transform expressing the displacement field of the elastic substrate as an integral over the traction field. The existence of this transform is used to derive a Bayesian method for converting noisy measurements of substratum displacement into ''images'' of the actual traction forces exerted by adherent or locomoting cells. Finally, the Monte Carlo validation of the statistical method is discussed, some new rheological studies of films are presented, and a practical application is given.