INVITRO TOXICITY OF TOPICAL ANTIMICROBIAL AGENTS TO HUMAN-FIBROBLASTS
INVITRO TOXICITY OF TOPICAL ANTIMICROBIAL AGENTS TO HUMAN-FIBROBLASTS
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DOI：
10.1016/0022-4804(89)90069-3
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发表时间：
1989-03-01
影响因子：
2.2
通讯作者：
HEGGERS, JP
中科院分区：
文献类型：
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作者：
MCCAULEY, RL;LINARES, HA;HEGGERS, JP
局部使用抗菌剂是最佳烧伤护理的关键。然而，将WI-38人二倍体成纤维细胞(ATCC CCL 75)和新鲜供体人皮肤成纤维细胞暴露于磺胺嘧啶银和马非尼德醋酸酯中，48小时内血细胞计数和总基质蛋白分析表明，细胞增殖显著降低。相差显微镜和透射电子显微镜观察到细胞形态的改变和细胞质细胞器和细胞核的进行性恶化。这些发现可能解释了使用局部抗菌药物后伤口愈合延迟的临床观察。
Topical antimicrobial agents are essential to optimal burn care. However, exposure of WI-38 human diploid fibroblasts (ATCC CCL 75) and fresh donor human dermal fibroblasts to silver sulfadiazine and mafenide acetate results in a significant reduction in cell proliferation, as determined by hemocytometer cell counts and total matrix protein assay, within 48 hr of exposure. Changes in cellular morphology and progressive deterioration of cytoplasmic organelles and the nucleus are seen with phase-contrast microscopy and transmission electron microscopy. These findings may explain the clinical observation of delayed wound healing after the use of topical antimicrobial agents.