HUMAN 18-S RIBOSOMAL-RNA SEQUENCE INFERRED FROM DNA-SEQUENCE - VARIATIONS IN 18-S SEQUENCES AND SECONDARY MODIFICATION PATTERNS BETWEEN VERTEBRATES
HUMAN 18-S RIBOSOMAL-RNA SEQUENCE INFERRED FROM DNA-SEQUENCE - VARIATIONS IN 18-S SEQUENCES AND SECONDARY MODIFICATION PATTERNS BETWEEN VERTEBRATES
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DOI：
10.1042/bj2320725
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发表时间：
1985-01-01
影响因子：
4.1
通讯作者：
MADEN, BEH
中科院分区：
文献类型：
--
作者：
MCCALLUM, FS;MADEN, BEH
我们已经确定的DNA序列编码18 S核糖体RNA在人和青蛙，爪蟾。我们还更正了非洲爪蟾18 S序列：序列中G-684后有一个A残基。这些和其他可用的数据提供了一些代表性的例子，在不同的脊椎动物群体之间的18 S核糖体RNA的一级结构和二级修饰的变化。首先，非洲爪蟾和非洲爪蟾18 S核糖体基因彼此不同，只有两个碱基替换，我们没有发现非洲爪蟾的18 S核糖体DNA内的种内异质性的证据（与非洲爪蟾转录间隔区相反）。第二，人类18 S序列与非洲爪蟾的18 S序列的差异约为100%。百分之六点五大约4%的差异是单碱基变化;其余的包括人类序列中的插入和影响几个核苷酸的其他变化。大多数这些更广泛的变化聚集在人类序列中核苷酸190和280之间的相对较短的区域中。第三，人类18 S序列与非灵长类哺乳动物序列仅相差约1%。第四，哺乳动物18 S核糖体RNA中几乎所有的47个甲基都可以定位在该序列中。甲基断裂与非洲爪蟾中的甲基断裂非常相似，但哺乳动物18 S核糖体RNA中有几个额外的甲基。最后，对人类和非洲爪蟾18 S核糖体RNA中假尿苷的估计数量进行了微小的修订。
We have determined the DNA sequences encoding 18 S ribosomal RNA in man and in the frog, Xenopus borealis. We have also corrected the Xenopus laevis 18 S sequence: an A residue follows G-684 in the sequence. These and other available data provide a number of representative examples of variation in primary structure and secondary modification of 18 S ribosomal RNA between different groups of vertebrates. First, Xenopus laevis and Xenopus borealis 18 S ribosomal genes differ from each other by only two base substitutions, and we have found no evidence of intraspecies heterogeneity within the 18 S ribosomal DNA of Xenopus (in contrast to the Xenopus transcribed spacers). Second, the human 18 S sequence differs from that of Xenopus by approx. 6.5%. About 4% of the differences are single base changes; the remainder comprise insertions in the human sequence and other changes affecting several nucleotides. Most of these more extensive changes are clustered in a relatively short region between nucleotides 190 and 280 in the human sequence. Third, the human 18 S sequence differs from non-primate mammalian sequences by only about 1%. Fourth, nearly all of the 47 methyl groups in mammalian 18 S ribosomal RNA can be located in the sequence. The methyl group disruption corresponds closely to that in Xenopus, but there are several extra methyl groups in mammalian 18 S ribosomal RNA. Finally, minor revisions are made to the estimated numbers of pseudouridines in human and Xenopus 18 S ribosomal RNA.