Engineering OsBAK1 gene as a molecular tool to improve rice architecture for high yield
Engineering OsBAK1 gene as a molecular tool to improve rice architecture for high yield
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DOI：
10.1111/j.1467-7652.2009.00444.x
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发表时间：
2009-10-01
影响因子：
13.8
通讯作者：
Chong, Kang
中科院分区：
文献类型：
--
作者：
Li, Dan;Wang, Lei;Chong, Kang
培育直立叶植物新品种是提高水稻产量的关键策略，因为具有这种性状的植物可以密植。直立叶是水稻植物中重要的形态性状，部分受油菜素内酯（BR）调节。迄今为止，只有少数基因可用于水稻分子育种。在这里，我们确定 OsBAK1 是改变水稻结构的潜在基因。基于水稻基因组序列，扩增出拟南芥BAK1（AtBAK1）基因的四个密切相关的同源物。系统发育分析和弱拟南芥突变体 bril-5 的抑制表明 OsBAK1 (Os08g0174700) 是 AtBAK1 的最近亲。遗传、生理和生化分析均表明 OsBAK1 的功能与 AtBAK1 相同。过度表达 OsBAK1 的截短胞内结构域，而不是 OsBAK1 的胞外结构域，会导致矮化表型，类似于水稻 BR 不敏感突变体植物。 OsBAK1的表达改变了水稻的重要农业性状，如株高、叶片直立度、籽粒形态特征和抗病反应。我们的结果表明，只需抑制 OsBAK1 的表达水平即可产生具有直立叶和正常繁殖的水稻新品种。因此，OsBAK1是一种潜在的分子育种工具，可通过改变水稻结构来提高水稻产量。
Generating a new variety of plant with erect-leaf is a critical strategy to improve rice grain yield, as plants with this trait can be dense-planted. The erect-leaf is a significant morphological trait partially regulated by Brassinosteroids (BRs) in rice plants. So far, only a few genes can be used for molecular breeding in rice. Here, we identified OsBAK1 as a potential gene to alter rice architecture. Based on rice genome sequences, four closely related homologs of Arabidopsis BAK1 (AtBAK1) gene were amplified. Phylogenetic analysis and suppression of a weak Arabidopsis mutant bril-5 indicated that OsBAK1 (Os08g0174700) is the closest relative of AtBAK1. Genetic, physiological, and biochemical analyses all suggest that the function of OsBAK1 is conserved with AtBAK1. Overexpression of a truncated intracellular domain of OsBAK1, but not the extracellular domain of OsBAK1, resulted in a dwarfed phenotype, similar to the rice BR-insensitive mutant plants. The expression of OsBAK1 changed important agricultural traits of rice such as plant height, leaf erectness, grain morphologic features, and disease resistance responses. Our results suggested that a new rice variety with erect-leaf and normal reproduction can be generated simply by suppressing the expression level of OsBAK1. Therefore, OsBAK1 is a potential molecular breeding tool for improving rice grain yield by modifying rice architecture.