Architecture of succinate dehydrogenase and reactive oxygen species generation
Architecture of succinate dehydrogenase and reactive oxygen species generation
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DOI：
10.1126/science.1079605
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发表时间：
2003-01-31
期刊：
影响因子：
56.9
通讯作者：
Iwata, S
中科院分区：
文献类型：
--
作者：
Yankovskaya, V;Horsefield, R;Iwata, S
大肠杆菌琥珀酸脱氢酶 (SQR) 的结构与线粒体呼吸复合物 II 类似，已被确定，揭示了从电子供体琥珀酸到末端电子受体泛醌的电子传递途径。研究发现，SQR 氧化还原中心的排列方式有助于防止黄素腺嘌呤二核苷酸形成活性氧 (ROS)。这可能是在有氧呼吸过程中表达 SQR 而不是相关酶富马酸还原酶的主要原因，后者会产生高水平的 ROS。此外，与线粒体 SQR 突变相关的遗传性疾病症状可能是酶中电子传输受损导致 ROS 形成的结果。
The structure of Escherichia coli succinate dehydrogenase (SQR), analogous to the mitochondrial respiratory complex II, has been determined, revealing the electron transport pathway from the electron donor, succinate, to the terminal electron acceptor, ubiquinone. It was found that the SQR redox centers are arranged in a manner that aids the prevention of reactive oxygen species (ROS) formation at the flavin adenine dinucleotide. This is likely to be the main reason SQR is expressed during aerobic respiration rather than the related enzyme fumarate reductase, which produces high levels of ROS. Furthermore, symptoms of genetic disorders associated with mitochondrial SQR mutations may be a result of ROS formation resulting from impaired electron transport in the enzyme.