Gene expression in grapevine cultivars in response to Bois Noir phytoplasma infection
Gene expression in grapevine cultivars in response to Bois Noir phytoplasma infection
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DOI：
10.1016/j.plantsci.2009.03.001
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发表时间：
2009-06-01
期刊：
影响因子：
5.2
通讯作者：
Pecchioni, Nicola
中科院分区：
文献类型：
--
作者：
Albertazzi, Giorgia;Milc, Justyna;Pecchioni, Nicola
黑树植物原体病是世界上一些地区葡萄的新发病害。目前还没有完全抗病的葡萄品种，对病害的生理机制也知之甚少。Affymetrix基因芯片(R)寡核苷酸阵列已被用于鉴定感染和恢复的霞多丽样品以及感染和健康的Manzoni Bianco样品之间的差异表达基因。在田间，曼佐尼表现出较轻的症状，而霞多丽则易患此病。结果表明，侵染植株中数百个基因的表达水平发生改变。对每个品种都有共同的和特定的影响，对各种代谢途径有影响。霞多丽的全光合链和光系统I活性、加尔文循环酶转录、脂质代谢和苯丙素生物合成均受到严重抑制。在受感染的霞多丽和曼佐尼植物中，观察到限制植物质体在植物中传播的物理障碍增加，负责细胞壁降解的基因受到抑制，而参与细胞壁强化的基因受到诱导。有趣的是，特别是在Manzoni中，Myb转录因子的表达被诱导，该转录因子属于一个在防御反应中起作用的基因家族。这是首次使用Affymetrix基因芯片(R)阵列分析葡萄与植物原体相互作用中的基因表达谱。提出的数据提供了一个有趣的图片，葡萄对黑树的转录反应，并允许选择几个候选基因为未来的功能分析。2009爱思唯尔爱尔兰有限公司版权所有。
Bois Noir phytoplasma is an emerging disease of Vitis vinifera in several regions of the world. No completely resistant grapevine cultivars are known and the physiology of disease remains still poorly understood so far.Affymetrix GeneChip (R) oligonucleotide arrays have been used to identify differentially expressed genes between infected and recovered samples from Chardonnay and between infected and healthy samples from Manzoni Bianco. In the field, Manzoni showed reduced symptoms, while Chardonnay was highly susceptible to the disease. Results showed that expression levels of few hundreds genes were altered in infected plants. both common and specific for each cultivar, with effects on various metabolic pathways. In Chardonnay a serious inhibition of whole photosynthetic chain and photosystem I activity, Calvin-cycle enzymes transcription, lipid metabolism and phenylpropanoid biosynthesis was observed. Increasing physical barriers to limit phytoplsma spread in the plant was observed in infected Chardonnay and Manzoni plants, with the repression of genes responsible for cell wall degradation and the induction of genes involved in cell wall reinforcement. Interestingly, specifically in Manzoni the expression of a Myb transcription factor, belonging to a gene family that has a role in defense response, was induced.This is the first analysis of gene expression profiling in a grapevine-phytoplasma interaction using Affymetrix GeneChip (R) array. Presented data provide an interesting picture of the transcriptional response of grapevine to Bois Noir and allowed the selection of several candidate genes for future functional analysis. (C) 2009 Elsevier Ireland Ltd. All rights reserved.