Genetic variability within isolates of Colletotrichum lindemuthianum belonging to race 65 from the state of Minas Gerais, Brazil
Genetic variability within isolates of Colletotrichum lindemuthianum belonging to race 65 from the state of Minas Gerais, Brazil
复制标题
DOI：
10.2478/s11756-008-0039-6
复制
发表时间：
2008-04-01
期刊：
影响因子：
1.5
通讯作者：
Davide, Livia M. C.
中科院分区：
文献类型：
--
作者：
Ishikawa, Francine H.;de Souza, Elaine A.;Davide, Livia M. C.
炭疽杆菌（Colletotrichum lindemuthianum）是普通豆（Phaseolus vulgaris）炭疽病的致病因子，具有广泛的遗传和致病变异性，这在抗性大豆品种的开发中引起了并发症。本研究的目的是通过随机扩增多态性DNA （RAPD）和吻合分析，确定巴西最常见的C. lindemuthianum 65种的变异。对巴西米纳斯吉拉斯州不同地区不同寄主品种、不同年份采集的13株65小种进行了研究。利用24条RAPD引物扩增出83条多态性条带。根据Sorensen-Dice系数估计遗传相似性，范围为0.54 ~ 0.82。聚类分析得到的树状图将分离菌株分为11个独立的类群。为了分析吻合性，当观察到不同菌株的菌丝融合时，分离株被认为是相容的。根据Russel & Rao系数估计每个分离株的相容反应比例，相似度估计范围为0.28 ~ 0.85。分离株可分为11个吻合群，其中10个吻合群仅由一个分离株形成。分离株LV61、LV73和LV58虽然属于同一吻合群，但RAPD分析表明它们在遗传上存在差异。RAPD和吻合分析的结果显示，林德菊种间存在很大的变异。
Colletotrichum lindemuthianum, the causal agent of anthracnose in the common bean (Phaseolus vulgaris), presents a wide genetic and pathogenic variability that gives rise to complications in the development of resistant bean cultivars. The aim of this study was to identify the variability within race 65 of C. lindemuthianum, the race most commonly encountered in Brazil, through randomly amplified polymorphic DNA (RAPD) and anastomosis analyses. Thirteen isolates of race 65, collected in different years and from various host cultivars located in diverse areas of the state of Minas Gerais, Brazil, were investigated. Twenty-four RAPD primers were employed and 83 polymorphic bands amplified. Genetic similarities were estimated from the Sorensen-Dice coefficient and ranged from 0.54 to 0.82. The dendrogram obtained by cluster analysis classified the isolates into 11 separate groups. For the purposes of the analysis of anastomosis, isolates were considered to be compatible when the fusion of hyphae from different isolates could be observed. The proportion of compatible reactions for each isolate was estimated and similarity estimates, based on the Russel & Rao coefficient, ranged from 0.28 to 0.85. Isolates were classified into 11 anastomosis groups, 10 of which were formed by only one isolate. Although isolates LV61, LV73 and LV58 were classified in the same anastomosis group, they were genetically distinct according to RAPD analysis. Results from both RAPD and anastomosis analyses revealed great variability within C. lindemuthianum race 65.