Isoflurane, but Not the Nonimmobilizers F6 and F8, Inhibits Rat Spinal Cord Motor Neuron CaV1 Calcium Currents.
Isoflurane, but Not the Nonimmobilizers F6 and F8, Inhibits Rat Spinal Cord Motor Neuron CaV1 Calcium Currents.
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DOI：
10.1213/ane.0000000000001111
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发表时间：
2016-03
影响因子：
5.7
通讯作者：
Blanck TJJ
中科院分区：
文献类型：
--
作者：
Recio-Pinto E;Montoya-Gacharna JV;Xu F;Blanck TJJ
挥发性麻醉药通过电压依赖性钙通道减少钙离子内流。钙离子影响神经递质的释放和神经元的兴奋性。由于挥发性麻醉剂特异性地作用于脊髓产生不动，我们观察了异氟烷和非固定剂F6(1，2-二氯六氟环丁烷)和F8(2，3-二氯八氟丁烷)对脊髓运动神经元和背根神经节神经元CaV1和CaV2钙通道的影响。采用膜片钳技术，比较了异氟醚与F6、F8对分离培养的成年大鼠脊髓运动神经元CaV1、CaV2通道以及成年大鼠背根神经节感觉神经元CaV1、CaV2通道的影响。在脊髓运动神经元中，异氟醚抑制通过CaV1通道的电流，而不是F6或F8。异氟醚和至少一种非阻滞剂抑制脊神经节细胞CaV1和CaV2通道电流，抑制脊髓运动神经元CaV2通道电流。异氟烷抑制脊髓运动神经元CaV1钙通道的结果与脊髓运动神经元可能介导异氟烷引起的静止的观点一致。还需要进一步的研究来检验脊髓运动神经元上CaV1钙电流的抑制是否足够，或者对其他通道/蛋白质的作用是否也有助于异氟醚诱导的不动。
Volatile anesthetics decrease Ca2+ entry through voltage-dependent Ca2+ channels. Ca2+ influences neurotransmitter release and neuronal excitability. Because volatile anesthetics act specifically on the spinal cord to produce immobility, we examined the effect of isoflurane and the nonimmobilizers F6 (1, 2- dichlorohexafluorocyclobutane) and F8 (2, 3- dichlorooctafluorobutane) on CaV1 and CaV2 Ca2+ channels in spinal cord motor neurons and dorsal root ganglion neurons. Using patch clamping, we compared the effects of isoflurane with those of F6 and F8 on CaV1 and CaV2 channels in isolated, cultured adult rat spinal cord motor neurons and on CaV1 and CaV2 channels in adult rat dorsal root ganglion sensory neurons. In spinal cord motor neurons, isoflurane, but not F6 or F8, inhibited currents through CaV1 channels. Isoflurane and at least one of the nonimmobilizers inhibited currents through CaV1 and CaV2 channels in dorsal root ganglion neurons and Cav2 in spinal cord motor neurons The findings that isoflurane, but not nonimmobilizers, inhibited CaV1 Ca2+ channels in spinal cord motor neurons are consistent with the notion that spinal cord motor neurons might mediate isoflurane-induced immobility. Additional studies are required to examine whether inhibition of CaV1 calcium currents in spinal cord motor neurons are sufficient, or whether actions on other channels/proteins also contribute to isoflurane-induced immobility.