Chromatin arranges in chains of mesoscale domains with nanoscale functional topography independent of cohesin.
Chromatin arranges in chains of mesoscale domains with nanoscale functional topography independent of cohesin.
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DOI：
10.1126/sciadv.aba8811
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发表时间：
2020-09
期刊：
影响因子：
13.6
通讯作者：
Schermelleh L
中科院分区：
文献类型：
--
作者：
Miron E;Oldenkamp R;Brown JM;Pinto DMS;Xu CS;Faria AR;Shaban HA;Rhodes JDP;Innocent C;de Ornellas S;Hess HF;Buckle V;Schermelleh L
先进的FIB-SEM和3D-SIM成像揭示了结构和功能模块中的粘着蛋白独立染色质排列。三维染色质组织在调节哺乳动物基因组功能中起着关键作用;然而，其在单细胞水平上的许多物理特征仍然未被探索。在这里，我们使用活细胞和固定细胞3D超分辨率和扫描电子显微镜来分析体细胞中的结构和功能核组织。我们确定了链互连的~200- 300 nm宽的染色质结构域（CD）组成的聚合核小体，可以与个别拓扑相关的结构域重叠，并从周围的RNA填充染色质间室不同。高含量的映射揭示了限制的凝聚素和活性组蛋白修饰的表面和丰富的抑制性修饰的核心CD在异染色质和常染色质区域。这种纳米级的功能拓扑结构在复制后的染色质中暂时放松，但在去除粘附素后仍然明显存在。我们的研究结果建立CD作为中尺度基因组组织的物理和功能模块。
Advanced FIB-SEM and 3D-SIM imaging uncover cohesin-independent chromatin arrangement into structural and functional modules. Three-dimensional (3D) chromatin organization plays a key role in regulating mammalian genome function; however, many of its physical features at the single-cell level remain underexplored. Here, we use live- and fixed-cell 3D super-resolution and scanning electron microscopy to analyze structural and functional nuclear organization in somatic cells. We identify chains of interlinked ~200- to 300-nm-wide chromatin domains (CDs) composed of aggregated nucleosomes that can overlap with individual topologically associating domains and are distinct from a surrounding RNA-populated interchromatin compartment. High-content mapping uncovers confinement of cohesin and active histone modifications to surfaces and enrichment of repressive modifications toward the core of CDs in both hetero- and euchromatic regions. This nanoscale functional topography is temporarily relaxed in postreplicative chromatin but remarkably persists after ablation of cohesin. Our findings establish CDs as physical and functional modules of mesoscale genome organization.
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影响因子：
3.4
作者：
Bruinsma, Robijn;Grosberg, Alexander Y.;Zidovska, Alexandra
通讯作者：
Zidovska, Alexandra
影响因子：
7.8
作者：
Chang, Chi-Lun;Weigel, Aubrey, V;Lippincott-Schwartz, Jennifer
通讯作者：
Lippincott-Schwartz, Jennifer
影响因子：
64.5
作者：
Bonev B;Mendelson Cohen N;Szabo Q;Fritsch L;Papadopoulos GL;Lubling Y;Xu X;Lv X;Hugnot JP;Tanay A;Cavalli G
通讯作者：
Cavalli G
DOI：
10.1186/s12964-016-0132-3
发表时间：
2016-04-06
期刊：
Cell communication and signaling : CCS
影响因子：
--
作者：
Järvelin AI;Noerenberg M;Davis I;Castello A
通讯作者：
Castello A
影响因子：
16.6
作者：
Chagin VO;Casas-Delucchi CS;Reinhart M;Schermelleh L;Markaki Y;Maiser A;Bolius JJ;Bensimon A;Fillies M;Domaing P;Rozanov YM;Leonhardt H;Cardoso MC
通讯作者：
Cardoso MC