Schisandrin B prevents ulcerative colitis and colitis-associated-cancer by activating focal adhesion kinase and influence on gut microbiota in an in vivo and in vitro model
Schisandrin B prevents ulcerative colitis and colitis-associated-cancer by activating focal adhesion kinase and influence on gut microbiota in an in vivo and in vitro model
复制标题
五味子 B 通过激活粘着斑激酶以及对体内和体外模型中肠道微生物群的影响来预防溃疡性结肠炎和结肠炎相关癌症
DOI：
10.1016/j.ejphar.2019.03.059
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发表时间：
2019-07-05
影响因子：
5
通讯作者：
Luo, Yan
中科院分区：
文献类型：
--
作者：
Li, Jiani;Lu, Yuan;Luo, Yan
结肠炎相关癌(CAC)与溃疡性结肠炎(UC)关系密切。五味子乙素(Schisandrin B，SCHB)治疗UC和CAC的疗效尚不清楚。我们观察了SCHB对UC葡聚糖硫酸钠(DSS)模型和CAC偶氮甲烷(AOM)/DSS模型的预防作用。此外，粘着斑激酶(FAK)的激活和对共生微生物区系的影响在UC的治疗中也是重要的。在体内和体外研究SCHB对UC发育过程中FAK激活的影响。我们还进行了16S rRNA测序，以检测SCHB对肠道微生物区系的调节。SCHB通过激活FAK而增强对肠上皮屏障的保护作用，这可能是因为SCHB的保护作用可通过抑制FAK的磷酸化而逆转。此外，SCHB对肠道微生物区系的影响也在UC的预防中发挥了重要作用。结果表明，SCHB通过加强对肠上皮屏障的保护和对肠道微生物区系的影响，从而抑制CAC，从而有效地预防UC。SCHB有望成为治疗UC和预防CAC的新方法。
Colitis-associated cancer (CAC) has a close relationship with ulcerative colitis (UC). Therapeutic effect of Schisandrin B (SchB) on UC and CAC remains largely unknown. We investigated the preventative effect of SchB on the dextran sulphate sodium (DSS) model of UC and azoxymethane (AOM)/DSS model of CAC. Furthermore, focal adhesion kinase (FAK) activation and influence on commensal microbiota are important for UC treatment. Impact on FAK activation by SchB in UC development was evaluated in vivo and vitro. We also conducted 16S rRNA sequencing to detect regulation of gut microbiota by SchB. Enhanced protection of intestinal epithelial barrier by SchB through activating FAK contributed to protective effect on colon for the fact that protection of SchB can be reversed by inhibition of FAK phosphorylation. Furthermore, influence on gut microbiota by SchB also played a significant role in UC prevention. Our results revealed that SchB was potent to prevent UC by enhancing protection of intestinal epithelial barrier and influence on gut microbiota, which led to inhibition of CAC. SchB was potential to become a new treatment for UC and prevention of CAC.