Identification of the minimum region of Bordetella pertussis Vag8 required for interaction with C1 inhibitor
Identification of the minimum region of Bordetella pertussis Vag8 required for interaction with C1 inhibitor
复制标题
百日咳博德特氏菌 Vag8 与 C1 抑制剂相互作用所需的最小区域的鉴定
DOI：
10.1111/1348-0421.12799
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发表时间：
2020
影响因子：
2.6
通讯作者：
Horiguchi Yasuhiko
中科院分区：
文献类型：
--
作者：
Onoda Naoki;Hiramatsu Yukihiro;Teruya Shihono;Suzuki Koichiro;Horiguchi Yasuhiko
百日咳博德特氏菌的一种自转运蛋白，毒力相关基因8（Vag 8），结合并灭活补体调节因子C1抑制剂（C1-Inh），并在逃避补体系统中发挥作用。然而，Vag 8和C1-Inh之间的分子相互作用仍不清楚。在这里，我们通过检查不同截短的Vag 8衍生物结合和抑制C1-Inh的能力，定位了与C1-Inh相互作用所需的Vag 8的最小区域。含有氨基酸残基102-548而非102-479和202-648的截短的Vag 8显示完整Vag 8的全部活性，表明Vag 8的单独的102-202和548-648氨基酸区域介导与C1-Inh的相互作用。
An autotransporter ofBordetella pertussis, virulence‐associated gene 8 (Vag8), binds and inactivates the complement regulator, C1 inhibitor (C1‐Inh), and plays a role in evasion of the complement system. However, the molecular interaction between Vag8 and C1‐Inh remains unclear. Here, we localized the minimum region of Vag8 required for interaction with C1‐Inh by examining the differently truncated Vag8 derivatives for the ability to bind and inactivate C1‐Inh. The truncated Vag8 containing amino‐acid residues 102–548, but not 102–479 and 202–648, showed the full activity of intact Vag8, suggesting that the separate 102–202 and 548–648 amino‐acid regions of Vag8 mediate the interaction with C1‐Inh.