THE ISOLATED PERFUSED-RAT-LIVER - CONCEPTUAL AND PRACTICAL CONSIDERATIONS
THE ISOLATED PERFUSED-RAT-LIVER - CONCEPTUAL AND PRACTICAL CONSIDERATIONS
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DOI：
10.1002/hep.1840060331
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发表时间：
1986-05-01
期刊：
影响因子：
13.5
通讯作者：
LARUSSO, NF
中科院分区：
文献类型：
--
作者：
GORES, GJ;KOST, LJ;LARUSSO, NF
尽管自Claude Bernard首次报道使用离体灌注大鼠肝脏（IPRL）以来已经过去了100多年（1），但该模型仍然是探索肝脏生理学和病理生理学的有价值和常用的工具。事实上，IPRL仍然是一个重要的实验模型，尽管有较新的技术（如肝切片，分离和培养的细胞，和分离的细胞器）用于评估肝功能。这种流行是由于这样的事实，即与uiuo模型（例如胆瘘大鼠）相比，IPRL允许灌注液的重复采样，允许肝脏容易地暴露于不同浓度的测试物质，并且适合于uiuo中不耐受的温度变化。此外，实验可以独立于其他器官系统、血浆成分和神经激素效应的影响进行。与其他体外模型（如分离和培养的肝细胞和分离的细胞器）相比，IPRL中保留了肝脏结构、细胞极性和胆汁流动。考虑到这些因素，更新并以选择性的方式审查IPRL似乎是及时的，特别是因为它涉及肝功能研究，模型不同技术方面的优点和缺点以及可靠评估器官活力的问题。这份手稿的目的不仅是帮助读者建立这种模式，而且也让他选择那些最适合他的特殊需要的条件。此外，它将提供足够的概念信息，使读者能够批判性地评估与IPRL的工作。然而，手稿并不意味着是一个全面的技术审查，因为这些都是现成的（2）。相反，它是一个高度选择性的更新，重点是概念，实际考虑和应用。
Although it has been over 100 years since Claude Bernard first reported the use of the isolated perfused rat liver (IPRL)(l), this model is still a valuable and commonly used tool for exploring the physiology and pathophysiology of the liver. Indeed, the IPRL remains an important experimental model despite the availability of newer techniques (eg, liver slices, isolated and cultured cells, and isolated organelles) for evaluating hepatic function. This popularity is due to the fact that, in contrast to in uiuo models, such as the bile fistula rat, the IPRL allows repeated sampling of the perfusate, permits easy exposure of the liver to different concentrations of test substances and is amenable to alterations in temperature that would not be tolerated in uiuo. Furthermore, experiments can be done independent of the influence of other organ systems, plasma constituents and neural-hormonal effects. In contrast to other in uitro models, such as isoiated and cultured hepatocytes and isolated organelles, hepatic architecture, cell polarity and bile flow are preserved in the IPRL. Given these considerations, it seems timely to update and, in a selective manner, to review the IPRL, especially as it relates to the study of hepatic function, the advantages and disadvantages of different technical aspects of the model and the problem of reliably assessing organ viability. This manuscript is intended not only to help the reader establish this model, but also to allow him to choose those conditions which best fit his particular needs. In addition, it will provide sufficient conceptual information to permit the reader to critically evaluate work with the IPRL. However, the manuscript is not meant to be a comprehensive technical review, as these are readily available (2). Rather, it is a highly selective update focusing on concepts, practical considerations and applications.