Enhanced platelet reactivity and thrombosis in Apoe-/- mice exposed to cigarette smoke is attenuated by P2Y12 antagonism
Enhanced platelet reactivity and thrombosis in Apoe-/- mice exposed to cigarette smoke is attenuated by P2Y12 antagonism
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DOI：
10.1016/j.thromres.2010.03.010
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发表时间：
2010-10-01
影响因子：
7.5
通讯作者：
Gairola, C. Gary
中科院分区：
文献类型：
--
作者：
Dong, Anping;Caicedo, Jessica;Gairola, C. Gary
吸烟增加急性动脉血栓形成的风险，包括心肌梗死，可能是由于对血管系统的多因素影响。血小板反应性升高可能是烟雾暴露的不良影响之一。方法：为了研究在动脉粥样硬化环境中烟雾暴露对血小板功能的影响，apoe缺陷的雌性小鼠，维持西方饮食，在全身暴露室中（4小时/天，5天/周）暴露于侧流香烟烟雾中12周。另一组野生型C57BL/6 J小鼠也以相同的方式暴露于烟雾中。结果：与暴露于过滤环境空气中的Apoe-/-小鼠相比，暴露于烟雾中的Apoe-/-小鼠显示出1.8 +/- 0.3倍的adp诱导的体外纤维蛋白原结合(P
Introduction: Smoking increases the risk of acute arterial thrombosis, including myocardial infarction, likely due to multi-factorial effects on the vasculature. Heightened platelet reactivity may be among the adverse effects of smoke exposure.Methods: To examine the effects of smoke exposure on platelet function in an atherosclerotic environment, Apoe-deficient female mice, maintained on a Western diet, were exposed (4 hrs/d, 5d/wk) to sidestream cigarette smoke in a whole-body exposure chamber for 12 weeks. A separate group of wild type C57BL/6 J mice were also exposed to smoke in an identical fashion.Results: In comparison to control Apoe-/- mice exposed to filtered ambient air, smoke-exposed Apoe-/- mice displayed a 1.8 +/- 0.3 fold enhanced ADP-induced fibrinogen binding ex vivo (P