Kaposi's sarcoma-associated herpesvirus latent and lytic gene expression as revealed by DNA arrays
Kaposi's sarcoma-associated herpesvirus latent and lytic gene expression as revealed by DNA arrays
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DOI：
10.1128/jvi.75.2.891-902.2001
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发表时间：
2001-01-01
影响因子：
5.4
通讯作者：
Kellam, P
中科院分区：
文献类型：
--
作者：
Jenner, RG;Albà, MM;Kellam, P
卡波西肉瘤相关疱疹病毒（KSHV；人类疱疹病毒 8）与卡波西肉瘤、原发性渗出性淋巴瘤 (PEL) 和多中心卡斯尔曼病这三种人类肿瘤有关。 KSHV 编码许多参与细胞周期、信号转导和宿主免疫反应调节的细胞蛋白同源物。在超过 85 个开放阅读框 (ORF) 的病毒补体中，只有少数的表达已被单独表征。我们构建了一个基于尼龙膜的 DNA 阵列，可以同时测量 KSHV 几乎每个 ORF 的表达。 PEL 衍生细胞系 BC-3 用于研究潜伏期和诱导裂解复制后 KSHV 的表达。聚类分析根据基因的表达谱对基因进行排列，揭示了表达与指定基因功能之间的相关性，这与疱疹病毒生命周期的已知阶段一致。此外，被认为在功能上相关的潜伏基因和裂解基因聚集成组。基因表达和功能之间的相关性也暗示了 KSHV 基因的可能作用尚未确定。
Kaposi's sarcoma-associated herpesvirus (KSHV; human herpesvirus 8) is associated,vith three human tumors, Kaposi's sarcoma, primary effusion lymphoma (PEL), and multicentric Castleman's disease. KSHV encodes a number of homologs of cellular proteins involved in the cell cycle, signal transduction, and modulation of the host immune response. Of the virus complement of over 85 open reading frames (ORFs), the expression of only a minority has been characterized individually. We have constructed a nylon membrane-based DNA array which allows the expression of almost every ORF of KSHV to be measured simultaneously. A PEL-derived cell line, BC-3, was used to study the expression of KSHV during latency and after the induction of lytic replication. Cluster analysis, which arranges genes according to their expression profile, revealed a correlation between expression and assigned gene function that is consistent with the known stages of the herpesvirus life cycle. Furthermore, latent and lytic genes thought to be functionally related cluster into groups. The correlation between gene expression and function also infers possible roles for KSHV genes yet to be characterized.