Adiponectin and its receptors in non-alcoholic steatohepatitis
Adiponectin and its receptors in non-alcoholic steatohepatitis
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DOI：
10.1136/gut.2003.037010
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发表时间：
2005-01-01
期刊：
影响因子：
24.5
通讯作者：
Tilg, H
中科院分区：
文献类型：
--
作者：
Kaser, S;Moschen, A;Tilg, H
背景资料：脂联素是一种脂肪细胞衍生的多肽，已被证明可以减轻非酒精性脂肪肝小鼠的脂肪变性和炎症。目的：在本研究中，我们希望确定接受减肥手术的病态肥胖患者的脂联素及其受体的肝脏表达。非酒精性脂肪性肝炎（NASH）患者或单纯性脂肪变性，以测试该系统的失调是否可能涉及这些疾病。患者和方法：采用荧光真实的时间聚合酶链反应分析了13例NASH患者和9例单纯性脂肪变性患者肝脏脂联素及其新近克隆的受体RI和RII（adipoRI和adipoRII）的mRNA表达。结果：NASH患者肝活检组织中脂联素和adipoRII mRNA的表达明显低于单纯脂肪变性患者，而adipoRII mRNA的表达无明显差异。在NASH中，adipoRII mRNA表达与血清天冬氨酸转氨酶水平、血清丙氨酸转氨酶水平和纤维化程度呈负相关。肝脂联素蛋白表达主要见于门静脉和肝窦内皮细胞，而adipoRII仅见于肝细胞。脂联素和adipoRII染色与NASH的受试者的活检组织中较低，与简单的脂肪atosization.Conclusion：脂联素和adipoRII的肝脏表达减少可能是非酒精性脂肪肝疾病的病理生理相关性。
Background: Adiponectin, an adipocyte derived polypeptide, has been shown to alleviate steatosis and inflammation in mice with non-alcoholic fatty liver disease.Aim: In the present study, we wished to define liver expression of adiponectin and its receptors in morbidly obese patients undergoing bariatric surgery.Patients with non-alcoholic steatohepatitis (NASH) or simple steatosis were investigated to test whether dysregulation of this system might be involved in these disorders. Patients and methods: Liver mRNA expression of adiponectin and its recently cloned receptors RI and RII (adipoRI and adipoRII) were analysed by fluorescence based real time polymerase chain reaction in 13 patients with NASH and nine with simple steatosis. Adiponectin and adipoRII protein expression were assessed by immunohistochemistry in a subgroup of patients.Results: Adiponectin and adipoRII mRNA expression were significantly reduced in liver biopsies of patients with NASH compared with simple steatosis while no difference was found in adipoRI mRNA expression. In NASH, adipoRII mRNA expression was negatively correlated with serum aspartate aminotransferase levels, serum alanine aminotransferase levels, and grade of fibrosis. Liver adiponectin protein expression was mainly found in endothelial cells of portal vessels and liver sinusoids whereas adipoRII expression was seen in hepatocytes only. Adiponectin and adipoRII staining were lower in biopsies of subjects with NASH compared with simple steatosis.Conclusion: Reduced hepatic expression of adiponectin and adipoRII might be of pathophysiological relevance in non-alcoholic fatty liver diseases.