RAPD grouping of accesses and cultivars of three Brachiaria species
RAPD grouping of accesses and cultivars of three Brachiaria species
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DOI：
10.4025/actasciagron.v30i4.5293
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发表时间：
2008-12-01
期刊：
影响因子：
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通讯作者：
Machado Neto, Nelson Barbosa
中科院分区：
文献类型：
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作者：
Ambiel, Ana Claudia;Guaberto, Luciana Machado;Machado Neto, Nelson Barbosa
本工作的目的是确定内和遗传间的相似性三臂形草种质资源访问和商业品种，使用RAID市场。种子用作DNA来源。从120个引物中筛选出10个十聚体引物，对107条多态性带进行了分子方差分析、Jaccard相似系数分析和种固定指数分析。24.4%的总变异包含在种间，种固定指数（FST）为0.24。该树表明，两个和75.6%的种内，与主要分支的形成：耳与三个外群种和另一个与种，后者分裂成三个小分支：一个与所有B。ruzizieusis样本;另一个样本全部为B。decumbens accessions和3个B. brizantha;最后一个有两个B。brizantha访问，所有商业品种和B。伏生蛇怪通过RAPD分析，可以对臂形草属植物的品种进行分类。物种间的遗传变异性指数被认为是低的，与自花受精物种相比有些低。B。brizantha的遗传变异最大，FST最低，说明哈特比遗传通量较高。
The aim of this work was to determine intra and intergenetic similarity among three Brachiaria species for germplasm accesses and commercial cultivars using RAID markets. Seeds were used as DNA source. 10 decamer 'primers' were selected among 120 'primers', and 107 polymorphic bands were used to perforin the analysis of molecular variance (Amova), Jaccard similarity coefficient and species fixation index. 24.4% of the total variability was contained between species a species fixation index (FST) of 0.24. The tree showed that two and 75.6% within species, with major branches were formed: otic with the three outgroup species and another with the species, the latter split into three minor branches: one with all B. ruzizieusis samples; another with all B. decumbens accessions and three of B. brizantha; and the last with two B. brizantha accesses, all commercial cultivars and B. decumbens cv. 'Basilisk'. It was possible to group, through RAPD, Brachiaria accesses and cultivars. The genetic variability index between species was considered low, and somewhat low when compared with autogamous species. B. brizantha showed the highest genetic variability and the lowest FST, which means that there is a higher Hart specific genetic flux than the others.