Degradation of 2,4-dihydroxybenzoate by Pseudomonas sp. BN9.
Degradation of 2,4-dihydroxybenzoate by Pseudomonas sp. BN9.
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假单胞菌对 2,4-二羟基苯甲酸酯的降解。
DOI：
10.1111/j.1574-6968.1993.tb06102.x
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发表时间：
1993
影响因子：
2.1
通讯作者：
H. Knackmuss
中科院分区：
文献类型：
--
作者：
A. Stolz;H. Knackmuss
研究了假单胞菌BN 9对2，4-二羟基苯甲酸的好氧降解。假单胞菌BN 9与2，4-二羟基苯甲酸盐一起生长的完整细胞氧化2，4-二羟基苯甲酸盐而不是水杨酸盐。加入NAD（P）H后，假单胞菌BN 9的无细胞提取物转化2，4-二羟基苯甲酸酯。部分纯化的蛋白质级分用NADH将2，4-二羟基苯甲酸酯转化为1，2，4-三羟基苯。1，2，4-三羟基苯被1，2-双加氧酶转化为马来酰丙酮酸，马来酰丙酮酸被NADH依赖性酶还原为3-氧代己二酸。假单胞菌（Pseudomonassp. BN 9）在2，4-二羟基苯甲酸盐的诱导下，产生了2，4-二羟基苯甲酸1-单加氧酶、1，2，4-三羟基苯1，2-双加氧酶和马来酰丙酮酸还原酶。
The aerobic degradation of 2,4-dihydroxybenzoate by Pseudomonas sp. BN9 was studied. Intact cells of Pseudomonas sp. BN9 grown with 2,4-dihydroxybenzoate oxidized 2,4-dihydroxybenzoate but not salicylate. Cell-free extracts of Pseudomonas sp. BN9 converted 2,4-dihydroxybenzoate after the addition of NAD(P)H. A partially purified protein fraction converted 2,4-dihydroxybenzoate with NADH to 1,2,4-trihydroxybenzene. 1,2,4-Trihydroxybenzene was converted by a 1,2-dioxygenase to maleylpyruvate, which was reduced by a NADH-dependent enzyme to 3-oxoadipate. 2,4-Dihydroxybenzoate 1-monooxygenase, 1,2,4-trihydroxybenzene 1,2-dioxygenase and maleylpyruvate reductase were induced in Pseudomonas sp. BN9 after growth with 2,4-dihydroxybenzoate.