Creation of a bioreactor for the application of variable amplitude mechanical stimulation of fibrin gel-based engineered cardiac tissue.
Creation of a bioreactor for the application of variable amplitude mechanical stimulation of fibrin gel-based engineered cardiac tissue.
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创建生物反应器，对基于纤维蛋白凝胶的工程心脏组织应用变幅机械刺激。
DOI：
10.1007/978-1-4939-1047-2_16
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Black3rd,LaurenD
中科院分区：
文献类型：
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作者：
Morgan,KathyY;Black3rd,LaurenD
本章详细介绍了三维纤维蛋白水凝胶作为工程心肌组织的创建，并介绍了一种机械拉伸生物反应器系统，该系统允许对结构进行周期到周期的可变幅度机械拉伸，作为调节结构使其更类似于天然组织的方法。尽管机械刺激已被确立为改善结构发育的标准方法，但大多数研究都是在恒定频率和恒定振幅下进行的，尽管变异性是健康心脏生理学的一个关键方面。在其他器官系统中引入变异性已证明对体外细胞功能有有益影响。我们假设在工程心脏组织中引入变异性可能具有类似的效果。
This chapter details the creation of three-dimensional fibrin hydrogels as an engineered myocardial tissue and introduces a mechanical stretch bioreactor system that allows for the cycle-to-cycle variable amplitude mechanical stretch of the constructs as a method of conditioning the constructs to be more similar to native tissue. Though mechanical stimulation has been established as a standard method of improving construct development, most studies have been performed under constant frequency and constant amplitude, even though variability is a critical aspect of healthy cardiac physiology. The introduction of variability in other organ systems has demonstrated beneficial effects to cell function in vitro. We hypothesize that the introduction of variability in engineered cardiac tissue could have a similar effect.