Rapamycin activates mammalian microautophagy
Rapamycin activates mammalian microautophagy
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DOI：
10.1016/j.jphs.2019.05.007
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发表时间：
2019-06-01
影响因子：
3.5
通讯作者：
Katsuki, Hiroshi
中科院分区：
文献类型：
--
作者：
Sato, Masahiro;Seki, Takahiro;Katsuki, Hiroshi
自噬-溶酶体蛋白水解分为宏观自噬（MA）、微观自噬（mA）和分子伴侣介导的自噬（CMA）。与MA和CMA相比，mA主要在酵母中进行研究。2011年，哺乳动物mA被鉴定为将胞质蛋白递送到多泡体中的途径。然而，其分子机制与酵母mA有很大不同。使用基于细胞的方法来评估mA和CMA，我们发现，雷帕霉素，酵母mA的激活剂，显着激活哺乳动物mA。虽然雷帕霉素激活MA，mA也激活雷帕霉素在MA缺陷细胞。这些发现表明，雷帕霉素是第一个确定的哺乳动物mA的激活剂。(C)2019作者由Elsevier B. V.代表日本药理学会制作和主办。
Autophagy-lysosome proteolysis is classified into macroautophagy (MA), microautophagy (mA) and chaperone-mediated autophagy (CMA). In contrast to MA and CMA, mA have been mainly studied in yeast. In 2011, mammalian mA was identified as a pathway to deliver cytosolic proteins into multivesicular bodies. However, its molecular mechanism is quite different from yeast mA. Using a cell-based method to evaluate mA and CMA, we revealed that rapamycin, an activator of yeast mA, significantly activated mammalian mA. Although rapamycin activates MA, mA was also activated by rapamycin in MA-deficient cells. These findings suggest that rapamycin is a first-identified activator of mammalian mA. (C) 2019 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society.