Repair of rat cranial bone defect by using bone morphogenetic protein-2-related peptide combined with microspheres composed of polylactic acid/polyglycolic acid copolymer and chitosan
Repair of rat cranial bone defect by using bone morphogenetic protein-2-related peptide combined with microspheres composed of polylactic acid/polyglycolic acid copolymer and chitosan
复制标题
骨形态发生蛋白2相关肽联合聚乳酸/聚乙醇酸共聚物和壳聚糖微球修复大鼠颅骨缺损
DOI：
10.1088/1748-6041/10/4/045004
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发表时间：
2015-08-01
影响因子：
4
通讯作者：
Xu, Shuyun
中科院分区：
文献类型：
--
作者：
Li, Jingfeng;Jin, Lin;Xu, Shuyun
研究骨形态发生蛋白2（BMP 2）相关肽P24和重组人BMP 2（rhBMP 2）复合聚乳酸-羟基乙酸共聚物（PLGA）/壳聚糖（CS）微球对大鼠颅骨缺损修复的促进作用。选取40只白色大鼠，平均分为4组（A组：rhBMP 2/PLGA/CS复合材料1 μg; B组：P24/PLGA/CS复合材料3 mg; C组：rhBMP 2 0.5 μg + P24/PLGA/CS复合材料1.5 mg; D组：空白PLGA/CS材料），建立直径为5 mm的大鼠颅骨缺损模型。将材料移植到颅骨缺损处。分别于术后6周和12周处死动物。进行放射学检查（X线成像和3D CT扫描）和组织学评价。测量颅骨缺损的修复面积，比较不同材料的成骨能力。颅骨组织学、影像学和修复面积测量显示，C组在两个时间点（6周和12周）的成骨效果优于A组和B组。A、B组疗效相似。D组颅骨缺损修复效果最差，可见大量纤维结缔组织。载rhBMP-2和P24的PLGA/CS复合微球具有良好的愈合性，并能相互促进成骨能力。rhBMP 2 + P24/PLGA/CS复合材料是一种新型的骨缺损修复材料，具有稳定的成骨活性。
The effects of the transplanted bone morphogenetic protein-2 (BMP2) -related peptide P24 and rhBMP2 combined with poly(lactic-co-glycolic acid) (PLGA)/chitosan (CS) microspheres were investigated in promoting the repair of rat cranial bone defect. Forty white rats were selected and equally divided into four groups (group A: 1 μg of rhBMP2/PLGA/CS composite; group B: 3 mg of P24/PLGA/CS composite; group C: 0.5 μg of rhBMP2 + 1.5 mg of P24/PLGA/CS composite; group D: blank PLGA/CS material), and rat cranial bone defect models with a diameter of 5 mm were established. The materials were transplanted to the cranial bone defects. The animals were sacrificed on weeks 6 and 12 post-operation. Radiographic examinations (x-ray imaging and 3D CT scanning) and histological evaluations were performed. The repaired areas of cranial bone defects were measured, and the osteogenetic abilities of various materials were compared. Cranial histology, imaging, and repaired area measurements showed that the osteogenetic effects at two time points (weeks 6 and 12) in group C were better than those in groups A and B. The effects in groups A and B were similar. Group D achieved the worst repair effect of cranial bone defects, where a large number of fibrous connective tissues were observed. The PLGA/CS composite microspheres loaded with rhBMP2 and P24 had optimal concrescence and could mutually increase their osteogenesis capability. rhBMP2 + P24/PLGA/CS composite is a novel material for bone defect repair with stable activity to induce bone formation.