Rhizopodin, a new compound from Myxococcus stipitatus (myxobacteria) causes formation of rhizopodia-like structures in animal cell cultures. Production, isolation, physico-chemical and biological properties.
Rhizopodin, a new compound from Myxococcus stipitatus (myxobacteria) causes formation of rhizopodia-like structures in animal cell cultures. Production, isolation, physico-chemical and biological properties.
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根足素是一种来自粘细菌（粘细菌）的新化合物，可在动物细胞培养物中引起根足样结构的形成。
DOI：
10.7164/antibiotics.46.741
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
H. Reichenbach
中科院分区：
文献类型：
--
作者：
F. Sasse;H. Steinmetz;G. Höfle;H. Reichenbach
从粘细菌柄粘球菌的培养液中分离到一种新的细胞抑制化合物根足素。该化合物抑制各种动物细胞培养物的生长而不杀死细胞。通过MTT测定法测量的ID 50为12约30 ng/ml，取决于细胞系。特别是成纤维细胞样生长的细胞表现出典型的形态学变化。它们变得更大，在一小时内就形成了长长的分枝和网状的流道。这些形态学变化是不可逆的。在K-562细胞中，Raspodin抑制水泡形成，因此可以通过与蛋白磷酸化相互作用而起作用。
A new cytostatic compound, rhizopodin, was isolated from the culture broth of the myxobacterium, Myxococcus stipitatus. The compound inhibited growth of various animal cell cultures without killing the cells. The ID50, measured by an MTT assay, was 12 approximately 30 ng/ml, depending on the cell line. Especially cells growing fibroblast-like showed typical morphological changes. They became larger and within hour formed long branching and reticular runners. These morphological changes were irreversible. Rhizopodin suppresses bleb formation in K-562 cells, and therefore could act by interacting with protein phosphorylation.