Effects of amino acids and albumin administration on albumin metabolism in surgically stressed rats: A basic nutritional study.
Effects of amino acids and albumin administration on albumin metabolism in surgically stressed rats: A basic nutritional study.
复制标题
氨基酸和白蛋白给药对手术应激大鼠白蛋白代谢的影响：一项基础营养研究。
DOI：
10.1002/jpen.2472
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Kuwahata M.
中科院分区：
文献类型：
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作者：
Wada A;Nakamura M;Kobayashi K;Kuroda A;Harada D;Kido S;Kuwahata M.
背景营养治疗和白蛋白制剂的施用在术后患者中很常见。然而，这些干预措施对白蛋白代谢的影响尚不清楚。我们阐明了术后白蛋白和/或肠外营养给药对其的影响。方法对Sprague-Dawley大鼠进行肠道摩擦手术，然后暴露肠道。随后，给他们注射实验溶液48小时，在24小时和48小时收集他们的血液样本，并在48小时切除肝脏。根据实验方案，将大鼠分为五组：非手术组（Non-surg）；葡萄糖和电解质溶液（GE）；氨基酸、葡萄糖和电解质溶液（AGE）； GE + 大鼠血清白蛋白（Alb）（GE + Alb）；和 AGE + Alb。他们的血浆白蛋白浓度；白蛋白分数合成率（ALB FSR）；巯基白蛋白/总白蛋白比率(MA比率)；并测量肝脏中白蛋白和肝细胞核因子-1（HNF-1）的信使RNA（mRNA）表达。结果GE和AGE组的白蛋白浓度显着下降。与 GE 组相比，AGE 组的 ALB FSR 显着增强。各组白蛋白 mRNA 表达量与 ALB FSR 相似，GE + Alb 组和 AGE + Alb 组 HNF-1 mRNA 表达量较非手术组显着降低。 AGE 组的 MA 比率与非手术组相似。 结论 术后给予包含肠外营养的氨基酸可增强 ALB FSR，并且仅在不同时给予白蛋白的情况下维持 MA 比率。
BackgroundNutrition therapy and administration of albumin preparations are common in postsurgical patients. However, the effects of these interventions on albumin metabolism are unclear. We elucidated the effect of postoperative albumin and/or parenteral nutrition administration on it.MethodsSprague‐Dawley rats underwent surgery involving intestinal rubbing followed by intestinal exposure. Subsequently, they were administered experimental solutions for 48 h, their blood samples were collected at 24 and 48 h, and livers were excised at 48 h. Based on experimental solutions, rats were divided into five groups: non‐surgical (Non‐surg); glucose and electrolyte solution (GE); amino acid, glucose, and electrolyte solution (AGE); GE + rat serum albumin (Alb) (GE + Alb); and AGE + Alb. Their plasma albumin concentrations; albumin fractional synthesis rate (ALB FSR); mercaptoalbumin/total albumin ratio (MA ratio); and messenger RNA (mRNA) expressions of albumin and hepatocyte nuclear factor‐1 (HNF‐1) in the liver were measured.ResultsThe GE and AGE groups showed significant decline in albumin concentrations. ALB FSR was significantly enhanced in the AGE group compared with the GE group. The mRNA expression of albumin was similar to ALB FSR in all groups and that of HNF‐1 was significantly decreased in the GE + Alb and AGE + Alb groups compared with the Non‐surg group. The MA ratio in the AGE group was similar to the Non‐surg group.ConclusionThe administration of amino acids comprising parenteral nutrition after surgery augmented ALB FSR and maintained the MA ratio only without simultaneous albumin administration.