CELL NUMBER AND DISTRIBUTION IN HUMAN AND RAT AIRWAYS
CELL NUMBER AND DISTRIBUTION IN HUMAN AND RAT AIRWAYS
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DOI：
10.1165/ajrcmb.10.6.8003339
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发表时间：
1994-06-01
影响因子：
6.4
通讯作者：
CRAPO, JD
中科院分区：
文献类型：
--
作者：
MERCER, RR;RUSSELL, ML;CRAPO, JD
使用形态测定程序来确定人和大鼠肺中气道的细胞数量、细胞体积、细胞直径和表面积。人支气管上皮细胞的细胞核大小明显大于其他肺细胞核。在支气管和细支气管中，人纤毛细胞核的平均体积分别为310 +/- 30 mum 3和167 +/- 12 mum 3。人细支气管的细胞核较小，与肺泡细胞的细胞核相当。在人支气管假复层上皮中，与纤毛细胞（1.1 +/- 0.1 mum 2）、杯状细胞（7.6 +/- 1.2 mum 2）或其他分泌细胞（12.0 +/- 2.1 mum 2）相比，基底细胞与基底膜接触的表面积较大（51.3 +/- 4.6 mum 2/细胞）。在气管远端的前四代气道中，基底细胞占人气道上皮细胞的30%，占大鼠气道上皮细胞的2%。在人和大鼠肺中，从气管到细支气管的总气道表面积分别为2，471 +/- 320和27.2 +/- 1.7 cm 2。这些气道表面积的直接测量值不到基于当前肺模型的估计值的一半。人和大鼠肺的气道上皮细胞总数分别为10.5 × 10（9）和0.05 × 10（9）。对于这两个物种，肺泡细胞比支气管上皮细胞多18倍。
Morphometric procedures were used to determine the number of cells, cell volume, cell diameter, and surface areas of the airways in human and rat lungs. Nuclear sizes of epithelial cells from human bronchi were significantly larger than other lung cell nuclei. The average volume of human ciliated cell nuclei was 310 +/- 30 mum3 and 167 +/- 12 mum3 in bronchi and bronchioles, respectively. The smaller nuclei of human bronchioles were comparable to those of alveolar cells. In the pseudostratified epithelium of human bronchi, basal cells had a large surface area in contact with the basement membrane (51.3 +/- 4.6 mum2 per cell) when compared with ciliated (1.1 +/- 0.1 mum2), goblet (7.6 +/- 1.2 mum2), or other secretory cells (12.0 +/- 2.1 mum2). In the first four airway generations distal to the trachea, basal cells account for 30% of the cells in human airway epithelium and 2% of the cells in rat airway epithelium. Total airway surface area from trachea to bronchioles was 2,471 +/- 320 and 27.2 +/- 1.7 cm2 in human and rat lungs, respectively. These direct measurements of airway surface area are less than half of the estimates based on current lung models. The total number of airway epithelial cells were 10.5 X 10(9) for human and 0.05 X 10(9) for rat lungs. For both species, there were 18 times more alveolar cells than bronchial epithelial cells.