Development and evaluation of 5-fluorouracil loaded chitin nanogels for treatment of skin cancer
Development and evaluation of 5-fluorouracil loaded chitin nanogels for treatment of skin cancer
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DOI：
10.1016/j.carbpol.2012.07.060
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发表时间：
2013-01-02
影响因子：
11.2
通讯作者：
Jayakumar, R.
中科院分区：
文献类型：
--
作者：
Sabitha, M.;Rejinold, N. Sanoj;Jayakumar, R.
本研究的重点是开发和评价5-氟尿嘧啶（5-FU）负载甲壳素纳米凝胶（FCNG）。它形成了良好的，稳定的水分散体与球形颗粒在120-140 nm的尺寸范围内，并显示pH响应性溶胀和药物释放。MTT法显示，FCNG在0.4- 2.0mg/mL浓度范围内对黑色素瘤（A375）有毒性作用，但对人皮肤成纤维细胞（HDF）的毒性较小。共聚焦分析显示两种细胞均摄取FCNG。从皮肤渗透实验，FCNG显示出与对照5-FU几乎相同的稳态通量，但发现FCNG在皮肤深层中的保留是FCNG的4-5倍。组织学评价显示，通过阳离子带电几丁质的相互作用，表皮角质层松动，没有观察到炎症迹象，因此FCNG可以是治疗皮肤癌的良好选择。(C)2012爱思唯尔有限公司保留所有权利。
This study focuses on development and evaluation of 5-fluorouracil (5-FU) loaded chitin nanogels (FCNGs). It formed good, stable aqueous dispersion with spherical particles in 120-140 nm size range and showed pH responsive swelling and drug release. The FCNGs showed toxicity on melanoma (A375) in a concentration range of 0.4-2.0 mg/mL, but less toxicity toward human dermal fibroblast (HDF) cells by MTT assay. Confocal analysis revealed uptake of FCNGs by both cells. From skin permeation experiments, FCNGs showed almost same steady state flux as that of control 5-FU but the retention in the deeper layers of skin was found to be 4-5 times more from FCNGs. Histopathological evaluation revealed loosening of the horny layer of epidermis by interaction of cationically charged chitin, with no observed signs of inflammation and so FCNGs can be a good option for treatment of skin cancers. (C) 2012 Elsevier Ltd. All rights reserved.