EXPERIMENTAL YERSINIA-PESTIS INFECTION IN RODENTS AFTER INTRA-GASTRIC INOCULATION AND INGESTION OF BACTERIA
EXPERIMENTAL YERSINIA-PESTIS INFECTION IN RODENTS AFTER INTRA-GASTRIC INOCULATION AND INGESTION OF BACTERIA
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DOI：
10.1128/iai.36.3.1160-1167.1982
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发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
ALMEIDA, A
中科院分区：
文献类型：
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作者：
BUTLER, T;FU, YS;ALMEIDA, A
研究了口腔鼠疫感染的发病机制。讨论了3种啮齿动物对鼠疫耶尔森菌胃内接种的易感性、感染的病理和进展以及对鼠疫耶尔森IA抗原的抗体反应。小家鼠、小鼠和家鼠胃内接种细菌的50%致死剂量分别为log10 = 6.32、5.46和9.62，比皮下注射获得的值至少高1000倍。接种。研究表明，当摄入饮用水中的细菌时，小肌杆菌也容易受到致命感染。镜下病理学结果与严重的全身感染一致。胃内接种后不同时间的组织定量培养显示，血液、肝脏和脾脏的感染先于派尔氏集结和中间间淋巴结的感染。粪便培养呈阴性。用于接种的鼠疫耶尔森氏菌菌株在暴露于pH≤1的缓冲溶液时被杀死。 3.在一些幸存的啮齿类动物灌胃后观察到抗体反应。因此，鼠疫耶尔森氏菌是明显有效的口腔病原体，可产生致命的全身感染和具有免疫力的自限性感染，但不会产生肠道病理学或导致细菌经粪便排泄。
The pathogenesis of oral plague infection was studied. The susceptibility of 3 species of rodents to intragastric inoculation of Y. pestis, the pathology and progression of infection and antibody responses to fraction IA antige of Y. pestis were discussed. The 50% lethal doses of bacteria by intragastric inoculation for Mus musculus, Zygodontomys pixuna and Rattus rattus were log10 = 6.32, 5.46 and 9.62, respectively, which were at least 1000-fold higher than the values obtained by s.c. inoculation. M. musculus was shown to be susceptible to lethal infection also when bacteria were ingested in drinking water. Microscopic pathology was consistent with heavy systemic infection. Quantitative cultures of tissues at different times after intragastric inoculation revealed that infections of blood, liver and spleen preceded infections of Peyer''s patches and mescenteric lymph nodes. Stool cultures were negative. The strain of Y. pestis used for inoculation was killed when exposed to a buffered solution at pH .ltoreq. 3. Antibody responses were observed in some of the surviving rodents after intragastric challenge. Thus, Y. pestis evidently effective oral pathogen that produced fatal systemic infections and self-limited infections with immunity but did not produce enteric pathology or lead fecal excretion of bacteria.