Regulation of surfactant protein D expression by glucocorticoids in vitro and in vivo.
Regulation of surfactant protein D expression by glucocorticoids in vitro and in vivo.
复制标题
糖皮质激素对体外和体内表面活性蛋白 D 表达的调节。
DOI：
10.1165/ajrcmb.10.1.8292379
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发表时间：
1994
影响因子：
6.4
通讯作者：
Shannon,JM
中科院分区：
文献类型：
--
作者：
Deterding,RR;Shimizu,H;Fisher,JH;Shannon,JM
SP-D 是最近描述的一种肺相关蛋白，由 II 型肺泡细胞产生，可能在肺宿主防御中发挥作用。由于对 SP-D 的激素调节知之甚少，并且已知其他表面活性蛋白（SP-A、SP-B 和 SP-C）受糖皮质激素调节，因此我们试图确定糖皮质激素对胎鼠肺中体外和体内 SP-D mRNA 和蛋白表达的影响。对妊娠第 15 天或第 18 天胎儿的肺外植体进行体外实验。外植体在有或没有氢化可的松的无血清条件下培养 3 天。通过Northern印迹分析评估SP-D mRNA表达。使用针对 SP-D 的多克隆抗体和标准免疫组织化学技术分析 SP-D 蛋白表达。与体内对照相比，SP-D mRNA 的表达在胎儿第 15 天外植体中增加，但在不添加氢化可的松培养的胎儿第 18 天外植体中保持不变。添加氢化可的松导致两个胎龄的 SP-D mRNA 表达增加。将 SP-D mRNA 表达模式与其他表面活性剂蛋白的表达进行比较，发现与 SP-B 最相似。体内实验是在胎儿第 15、16 和 17 天时母体给予地塞米松 (1 mg/kg) 或等体积的生理盐水，或在胎儿第 17 天并在胎儿第 18 天处死进行。（摘要截短为 250 字）
SP-D is a recently described lung-associated protein that is produced by alveolar type II cells and may function in pulmonary host defenses. Since little is known regarding the hormonal regulation of SP-D, and since the other surfactant proteins (SP-A, SP-B, and SP-C) are known to be regulated by glucocorticoids, we sought to determine the effects of glucocorticoids on SP-D mRNA and protein expression, both in vitro and in vivo, in the fetal rat lung. In vitro experiments were performed on lung explants from fetuses on gestational day 15 or 18. Explants were cultured in serum-free conditions with or without hydrocortisone for 3 days. SP-D mRNA expression was evaluated by Northern blot analysis. SP-D protein expression was analyzed using a polyclonal antibody against SP-D and standard immunohistochemical techniques. The expression of SP-D mRNA increased in fetal day 15 explants but remained unchanged in fetal day 18 explants cultured without the addition of hydrocortisone, compared with in vivo controls. The addition of hydrocortisone resulted in increases in SP-D mRNA expression at both gestational ages. This pattern of SP-D mRNA expression was compared with the expression of the other surfactant proteins and found to be most similar to that of SP-B. In vivo experiments were performed using maternal administration of dexamethasone (1 mg/kg) or an equal volume of saline on fetal days 15, 16, and 17 or on fetal day 17 with sacrifice on fetal day 18.(ABSTRACT TRUNCATED AT 250 WORDS)