The application of thionine-graphene nanocomposite in chiral sensing for Tryptophan enantiomers
The application of thionine-graphene nanocomposite in chiral sensing for Tryptophan enantiomers
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硫堇-石墨烯纳米复合材料在色氨酸对映体手性传感中的应用
DOI：
10.1016/j.bioelechem.2013.09.002
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发表时间：
2013-12-01
影响因子：
5
通讯作者：
Fu, Yingzi
中科院分区：
文献类型：
--
作者：
Guo, Liju;Zhang, Qing;Fu, Yingzi
成功地合成了硫氨酸-石墨烯(Thi-GR，带正电荷)纳米复合材料，作为DS-DNA良好的生物相容性基质，作为手性选择剂，在铜(11)的辅助下构建了色氨酸(Trp)对映体的电化学手性生物传感器。纳米仿生界面的构建如下：首先，将纳米复合材料滴在玻碳电极(GCE)表面，然后通过反电荷吸附技术将dsDNA(带负电荷)固定在纳米复合膜上。用扫描电子显微镜(SEM)、紫外-可见光谱(UV-Vis)和循环伏安(CV)对该生物功能化纳米复合材料进行了表征。利用手性生物传感器研究了循环伏安法对双链DNA和色氨酸对映体的识别效果。结果表明，当铜(II)存在时，L-色氨酸对映体有较大的电化学反应，表明该方法可用于对色氨酸对映体的选择性识别。在最佳条件下，手性生物传感器对色氨酸对映体在5.0×10~(-4)~2.5 mM范围内与色氨酸对映体呈良好的线性关系，检测下限为0.17µM(S/N=3)。计算出[Cu(II)(L-Trp)(2)]和[Cu(II)(L-Trp)(2)]的结合常数分别为2.97×10(3)M-1和2.50×10(2)M-1。(C)2013爱思唯尔B.V.保留所有权利。
The thionine-graphene (THi-GR, positively charged) nanocomposite was successfully synthesized as a good biocompatible matrix for ds-DNA which acted as a chiral selector to construct an electrochemical chiral biosensor for Tryptophan (Trp) enantiomers sensing with the assistance of Cu(ll). The nano-bionic interface was constructed as follows: Firstly, the nanocomposite was dropped on the surface of glassy carbon electrode (GCE), and then dsDNA (negatively charged) was immobilized onto the nanocomposite film via the opposite-charged adsorption techniques. This biofunctionalized nanocomposite was characterized with scanning electron microscopy (SEM), ultraviolet-visible (UV-vis) spectrometry and cyclic voltammetry (CV). The chiral biosensor was employed to study the recognition effect between ds-DNA and Trp enantiomers by CV. The results show that larger electrochemical response was obtained from L-Trp when Cu(II) was present, indicating this strategy could be employed to enantioselectively recognize Trp enantiomers. Under optimum conditions, the chiral biosensor exhibited a good linear response to Trp enantiomers in the range of the concentration of [Cu(II)(Trp)(2)] from 5.0 x 10(-4) to 2.5 mM with a low limit of detection of 0.17 mu M (S/N = 3). The binding constant was calculated to be 2.97 x 10(3) M-1 for [Cu(II)(L-Trp)(2)] and 2.50 x 10(2) M-1 for [Cu(II)(L-Trp)(2)]. (C) 2013 Elsevier B.V. All rights reserved.