Cell motility and SCAR localisation in axenically growing Dictyostelium cells
Cell motility and SCAR localisation in axenically growing Dictyostelium cells
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DOI：
10.1016/j.ejcb.2006.05.014
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发表时间：
2006-09-01
影响因子：
6.6
通讯作者：
Insall, Robert H.
中科院分区：
文献类型：
--
作者：
Pollitt, Alice Y.;Blagg, Simone L.;Insall, Robert H.
网骨藻是一种受欢迎的实验生物，特别是用于肌动蛋白动力学、细胞运动和趋化性的研究。我们发现无菌细胞在生长过程中的运动性出乎意料地不同于其他菌株。特别是，营养AX3细胞不显示可检测的本地化的SCAR及其监管复杂的肌动蛋白丰富的突起，如丝状伪足和伪足。类似地，一系列不同的突变，特别是SCAR复合物和Ras蛋白的成员的敲除，在不同亲本菌株的营养生长期间引起不同的表型。发育逆转了这种不寻常的行为;具有聚集能力的AX3细胞以与其他菌株和物种的细胞相同的方式定位SCAR。因此，使用营养细胞进行的细胞运动性研究应谨慎解释。(c)2006年Elsevier GmbH。All rights reserved.
Dictyostelium is a popular experimental organism, in particular for studies of actin dynamics, cell motility and chemotaxis. We find that the motility of axenic cells is unexpectedly different from other strains during growth. In particular, vegetative AX3 cells do not show detectable localisation of SCAR and its regulatory complex to actin-rich protrusions such as filopodia and pseudopodia. Similarly, a range of different mutations, in particular knockouts of members of the SCAR complex and Ras proteins, cause different phenotypes during vegetative growth in different parental strains. Development reverses this unusual behaviour; aggregation-competent AX3 cells localise SCAR in the same way as cells of other strains and species. Studies on cell motility using vegetative cells should therefore be interpreted with caution. (c) 2006 Elsevier GmbH. All rights reserved.