POLLEN PROTEASES - THEIR POTENTIAL ROLE IN INSECT DIGESTION
POLLEN PROTEASES - THEIR POTENTIAL ROLE IN INSECT DIGESTION
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DOI：
10.1016/0020-1790(79)90011-8
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
HUNT, JH
中科院分区：
文献类型：
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作者：
GROGAN, DE;HUNT, JH
在体外分析了蜜蜂访问过的植物类群[黄萱草、短毛草、苹果、刺槐、加拿大茜草、紫花木槿、牡丹、佛罗里达山茱萸、金银花、蒲公英、菊苣和向日葵]花粉中4种酶活性的存在：胰蛋白酶样、凝乳胰蛋白酶样、羧肽酶a样和羧肽酶b样。所有被检查的花粉至少含有胰凝乳活性；其他活动发生不规律。所发现的酶的数量被制成表格，并与蜜蜂中肠相同的4种酶的活性水平进行比较。蜜蜂中肠的活性水平超过花粉的活性水平，但在某些情况下花粉酶似乎足以产生可能的生理活性。
Pollens of plant taxa [Hemerocallis fulva, Philadelphus pubescens, Malus sp., Robinia pseudoacacia, Cercis canadensis, Hibiscus syriacus, Peunia sp., Cornus florida, Campsis radicans, Lonicera sp., Taraxacum sp., Cichorium intybus and Helianthus tuberosus] known to be visited by bees were analyzed in vitro for the presence of 4 enzymic activities: trypsin-like, chymotrypsin-like, carboxypeptidase A-like, and carboxypeptidase B-like. All pollens examined contained at least chymotryptic activity; other activities were irregular in occurrence. Quantities of the enzymes found are tabulated and are compared with levels of the same 4 enzymic activities of the midguts of Apis mellifera L. Honeybee midgut activity levels exceed those of the pollens, but the pollen enzymes seem sufficient in some cases for probable physiological activity.