EFFECT OF MACROPHAGES ON PROLIFERATION OF GRANULOSA-CELLS IN THE OVARY IN RATS
EFFECT OF MACROPHAGES ON PROLIFERATION OF GRANULOSA-CELLS IN THE OVARY IN RATS
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DOI：
10.1530/jrf.0.0960241
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发表时间：
1992-09-01
期刊：
影响因子：
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通讯作者：
OKAMURA, H
中科院分区：
文献类型：
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作者：
FUKUMATSU, Y;KATABUCHI, H;OKAMURA, H
在促性腺激素引发的未成熟雌性大鼠中检查了巨噬细胞对颗粒细胞增殖的影响。使用小鼠抗大鼠巨噬细胞单克隆抗体TRPM-2和TRPM-3，在卵泡的颗粒层和窦以及卵泡周围的黄体和间质中观察到巨噬细胞。 TRPM-2阳性细胞和TRPM-3阳性细胞之间的分布没有差异。在生长的卵泡中也发现了具有一些不同大小的细胞质液泡的巨噬细胞。窦前、窦和成熟卵泡中巨噬细胞与颗粒细胞的平均比率分别为0.008、0.007和0.002。与腹腔巨噬细胞一起培养的颗粒细胞的[H-3]胸苷标记明显增多，当巨噬细胞与颗粒细胞的比例为0.01时，标记指数达到峰值25.0%，而单独培养颗粒细胞时的标记指数为14.2%。巨噬细胞与颗粒细胞的比例与体内窦前和窦卵泡中的比例相似。这些结果表明巨噬细胞作为生长卵泡中的局部介质参与促进颗粒细胞的增殖。
The effect of macrophages on proliferation of granulosa cells was examined in gonadotrophin-primed immature female rats. The mouse anti-rat macrophage monoclonal antibodies TRPM-2 and TRPM-3 were used and macrophages were observed in the granulosa layer and antrum of follicles and in corpora lutea and stroma around follicles. There was no difference in distribution between TRPM-2-positive cells and TRPM-3-positive cells. Macrophages with some cytoplasmic vacuoles of various sizes were also demonstrated in growing follicles. The average ratios of macrophages to granulosa cells in preantral, antral and mature follicles were 0.008, 0.007 and 0.002, respectively. Labelling with [H-3]thymidine of granulosa cells cultured with peritoneal macrophages was significantly greater and the labelling index peaked to 25.0% when the ratio of macrophages to granulosa cells was 0.01, compared with the value of 14.2% when the granulosa cells were cultured alone. This ratio of macrophages to granulosa cells was similar to that in the preantral and antral follicles in vivo. These results suggest that macrophages participate in promoting proliferation of granulosa cells as local mediators in growing follicles.