Change in the mode of gene expression of the hypopharyngeal gland cells with an age-dependent role change of the worker honeybee Apis mellifera L.
Change in the mode of gene expression of the hypopharyngeal gland cells with an age-dependent role change of the worker honeybee Apis mellifera L.
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DOI：
10.1111/j.1432-1033.1997.t01-1-00797.x
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发表时间：
1997-11-01
期刊：
影响因子：
--
通讯作者：
Kubo, T
中科院分区：
文献类型：
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作者：
Ohashi, K;Natori, S;Kubo, T
工蜂下咽腺体合成的主要蛋白质随工蜂年龄的变化由蜂乳蛋白转变为α-葡萄糖苷酶。以前，我们证明了α-葡萄糖苷酶基因在觅食蜂腺中特异表达[Ohashi，K.，Sawata，M.，Takeuchi，H.，Natori，S.&Kubo，T.(1996)Biochem。生物群落。[中英文摘要]Re.Commun.221、380-385]。在这里，我们描述了两个蜂奶56 kDa和kDa蛋白的cDNA的分离和分析。56 kDa蛋白是一种糖蛋白，与蜂王浆相关蛋白57-1(pRJP57-1)和pRJP57-2编码的蛋白的氨基酸序列同源性分别为63.2%和56.9%。获得的-kDa蛋白与pRJP57-1完全相同。因此，这些蜂奶蛋白似乎形成了一个结构上相关的蛋白质家族。-kDa蛋白/RJP57-1基因在哺乳蜂腺中特异表达，而56-kDa蛋白在哺乳蜂腺和觅食蜂腺中均有表达。用原位杂交方法在哺乳蜂腺的整个腺泡中检测到56-kDa和-kDa蛋白的mRNAs，而在觅食蜂腺的腺泡中检测到56 kDa蛋白和cc-葡萄糖苷酶的mRNAs。因此，随着工蜂年龄的变化，下咽腺腺泡的单个分泌细胞表达这些基因的方式也不同。
Major proteins synthesized in the hypopharyngeal gland of the worker honeybee change from bee-milk proteins to alpha-glucosidase in accordance with the age-dependent role change of the worker bee. Previously, we showed that the gene for alpha-glucosidase is expressed specifically in the forager-bee gland [Ohashi, K., Sawata, M., Takeuchi, H., Natori, S. & Kubo, T. (1996) Biochem. Biophys. Res. Commun. 221, 380-385]. Here, we describe the isolation and analysis of cDNAs for two bee-milk 56-kDa and 64-kDa proteins. The 56-kDa protein was a glycoprotein which shared 63.2% and 56.9% amino acid sequence identities with proteins encoded by cDNA for royal-jelly-related protein 57-1 (pRJP57-1) and pRJP57-2. The 64-kDa protein cDNA was identical to pRJP57-1. Thus, these bee-milk proteins seem to form a structurally related protein family. The gene for the 64-kDa protein/RJP57-1 was expressed specifically in the nurse-bee gland, whereas that for the 56-kDa protein was expressed in both the nurse-bee and forager-bee glands. mRNAs for the 56-kDa and 64-kDa proteins were detected by in situ hybridization in a whole acinus of the nurse-bee gland, whereas mRNAs for the 56-kDa protein and cc-glucosidase were detected in that of the forager-bee gland. Therefore, the individual secretory cells of the acinus of the hypopharyngeal gland were shown to express these genes differently with the age-dependent role change of the worker bee.