ANTIGENIC MAP OF THE RAT CEREBELLAR CORTEX - THE DISTRIBUTION OF PARASAGITTAL BANDS AS REVEALED BY MONOCLONAL ANTI-PURKINJE CELL ANTIBODY MABQ113
ANTIGENIC MAP OF THE RAT CEREBELLAR CORTEX - THE DISTRIBUTION OF PARASAGITTAL BANDS AS REVEALED BY MONOCLONAL ANTI-PURKINJE CELL ANTIBODY MABQ113
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DOI：
10.1002/cne.902560104
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发表时间：
1987-02-01
影响因子：
2.5
通讯作者：
LECLERC, N
中科院分区：
文献类型：
--
作者：
HAWKES, R;LECLERC, N
解剖学和生理学的映射方法都揭示了哺乳动物小脑皮质由一个家族的旁神经带细胞组成，每个带都有自己的传入和传出轴突模式。单克隆抗体mabQ 113识别一种未知的多肽抗原，该抗原仅限于大鼠小脑浦肯野细胞的一个亚群。免疫反应细胞排列成延伸至整个蚓部和半球的旁带状物。单个浦肯野细胞表达Q113表位可能不是全有或全无的，因为蚓部的条带往往比半球的染色更强烈。条带显示关于中线对称，并且个体与个体之间可再现。整体包埋免疫细胞化学和连续重建显示mabQ 113+浦肯野细胞的中间带邻近中线（P1+）和其他六个阳性带对称地分布在两侧（P2+至P7+）。在大部分皮质中，带状物是明显的，但倾向于在腹侧和尾侧融合。个体间差异有两个来源。首先，大多数动物在蚓部表达多余的“卫星”带。卫星带通常是一个细胞宽，不是两侧对称的，并且个体与个体之间在位置和数量上不同。第二，单个条带的精确位置可能不同，这可能取决于皮质分叶的不同，例如，P4+在小叶VIII/IX中的位置和P6+在小叶VII中的位置。虽然寄生虫带的计划是一个很好的描述蠕虫组织，分布mabQ 113+和mabQ 113-浦肯野细胞在半球可能更好地描述为棋盘状的抗原补丁。
Both anatomical and physiological mapping methods have revealed that the mammalian cerebellar cortex consists of a family of parasagittal bands of cells, each band with its own pattern of afferent and efferent axons. Monoclonal antibody mabQ113 recognizes an unknown polypeptide antigen that is confined to a subset of rat cerebellar Purkinje cells. Immunoreactive cells are arranged into parasagittal bands extending throughout the vermis and hemispheres. Expression of the Q113 epitope by individual Purkinje cells may not be all-or-nothing, since the bands tend to be more strongly stained in the vermis than the hemispheres. The band display is symmetrical about the midline and reproducible from individual to individual. Whole-mount immunocytochemistry and serial reconstruction reveal a median band of mabQ113+ Purkinje cells adjacent to the midline (P1+) and six other positive bands disposed symmetrically at either side (P2+ to P7+). Bands are distinct throughout most of the cortex but tend to fuse ventrally and caudally. There are two sources of interindividual differences. Firstly, most animals express supernumerary "satellite" bands in the vermis. Satellite bands are usually one cell wide, are not bilaterally symmetrical, and differ in position and number from individual to individual. Secondly, the precise position of an individual band can differ, perhaps according to the variable cortical lobulation, for example, the position of P4+ in lobules VIII/IX and P6+ in lobule VII. While a scheme of parasagittal bands is a good description of the vermian organization, the distribution of mabQ113+ and mabQ113- Purkinje cells in the hemispheres may be better described as a checkerboard of antigenic patches.