Prostaglandin F(2alpha) regulates cytokine responses of mast cells through the receptors for prostaglandin E.
Prostaglandin F(2alpha) regulates cytokine responses of mast cells through the receptors for prostaglandin E.
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DOI：
10.1016/j.bbrc.2008.01.002
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发表时间：
2008-03
影响因子：
3.1
通讯作者：
Izumi Kaneko;T. Hishinuma;Kaori Suzuki;Y. Owada;Noriko Kitanaka;H. Kondo;J. Goto;H. Furukawa;M. Ono
中科院分区：
文献类型：
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作者：
Izumi Kaneko;T. Hishinuma;Kaori Suzuki;Y. Owada;Noriko Kitanaka;H. Kondo;J. Goto;H. Furukawa;M. Ono
越来越多的证据表明前列腺素类药物可调节肥大细胞功能并导致过敏性炎症的发生。本研究旨在确定前列腺素 (PG) F2α 在肥大细胞激活中的未确定功能及其所涉及的信号机制。通过液相色谱/串联质谱法同时定量前列腺素，揭示了骨髓源性肥大细胞 (BMMC) 中 PGF2α、血栓素 B2 和 6-酮-PGF1α 的组成型释放。脂多糖激活 BMMC 后，当培养物中补充 PGF2α 时，BMMC 中细胞因子的产生会增强。然而，在 BMMC 中未检测到 F 前列腺素受体（PGF2α 的选择性受体）。使用前列腺素受体拮抗剂进行的进一步研究揭示了另一种机制，其中 PGE 种类的受体（E 前列腺素受体）介导 BMMC 中的 PGF2α 信号。本研究提供了对 PGF2α 的新功能的深入了解，即肥大细胞激活的自分泌加速器。
There is an increasing body of evidence that prostanoids modulate mast cell functions and contribute to the development of allergic inflammation. The present study aimed to identify an undetermined function of prostaglandin (PG) F2αin mast cell activation and the signaling mechanism involved in it. Simultaneous quantification of prostanoids by liquid chromatography/tandem mass spectrometry revealed the constitutive release of PGF2α, thromboxane B2, and 6-keto-PGF1αfrom bone marrow-derived mast cells (BMMCs). Upon activation of BMMCs by lipopolysaccharide, the cytokine production in BMMCs was enhanced when the culture was supplemented with PGF2α. However, F prostanoid receptor—a selective receptor for PGF2α—was not detected in BMMCs. Further investigations performed using prostanoid receptor antagonists revealed an alternative mechanism wherein the receptors for PGE species—E prostanoid receptors—mediated the PGF2αsignal in BMMCs. The present study provides an insight into a novel function of PGF2α, i.e., an autocrine accelerator for mast cell activation.