Isolation and partial characterization of a lentivirus from talapoin monkeys (Myopithecus talapoin)
Isolation and partial characterization of a lentivirus from talapoin monkeys (Myopithecus talapoin)
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DOI：
10.1006/viro.1999.9794
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发表时间：
1999-07-20
期刊：
影响因子：
3.7
通讯作者：
Bosch, ML
中科院分区：
文献类型：
--
作者：
Osterhaus, ADME;Pedersen, N;Bosch, ML
我们发现了一种在塔拉普猴（Myopithecus talapoin）中普遍存在的新型慢病毒，扩展了之前对这些猴子血清中人类免疫缺陷病毒-1交叉反应性抗体的观察结果。我们从最初提供给我们的三只血清反应呈阳性的猴子中的一只获得了病毒分离物。该病毒暂定名为来自塔拉普猴的猿猴免疫缺陷病毒（SIVtal）。尽管分离这种病毒很困难，但它很容易通过未知的传播途径在圈养的猴子之间传播。该病毒可以在塔拉普猴的外周血单核细胞中短期繁殖，但不能在人外周血单核细胞或人 T 细胞系中繁殖。繁殖的病毒被用来感染一只幼稚塔拉普猴、四只恒河猴（M. mulatta）和两只食蟹猴（M. fasciculis）。所有动物都发生了血清转化，并且在实验感染后的短时间内可以重新分离出病毒。对感染 SIVtal 的圈养塔拉普猴的调查显示，长期（>2 年）感染动物的 CD4+ 细胞数量相对减少。在任何受感染的动物中均未观察到其他免疫缺陷迹象。 PCR 扩增随后对聚合酶基因的两个片段进行 DNA 测序表明，SIVtal 与目前已知的慢病毒不同，并且可能与赛克斯猴的 SIV 最相关。 (C) 1999 年学术出版社。
We have identified a novel lentivirus prevalent in talapoin monkeys (Myopithecus talapoin), extending previous observations of human immunodeficiency virus-1 cross- reactive antibodies in the serum of these monkeys. We obtained a virus isolate from one of three seropositive monkeys initially available to us. The virus was tentatively named simian immunodeficiency virus from talapoin monkeys (SIVtal). Despite the difficulty of isolating this virus, it was readily passed between monkeys in captivity through unknown routes of transmission. The virus could be propagated for short terms in peripheral blood mononuclear cells of talapoin monkeys but not in human peripheral blood mononuclear cells or human T cell lines. The propagated virus was used to infect a naive talapoin monkey, four rhesus macaques (M. mulatta), and two cynomolgus macaques (M. fascicularis). All animals seroconverted and virus could he reisolated during a short period after experimental infection. A survey of SIVtal-infected captive talapoin monkeys revealed a relative decrease in CD4+ cell numbers in chronically (>2 years) infected animals. No other signs of immunodeficiency were observed in any of the infected animals. PCR amplification followed by DNA sequencing of two fragments of the polymerase gene revealed that SIVtal is different from the presently known lentiviruses and perhaps most related to the SIV from Sykes monkeys. (C) 1999 Academic Press.