Toward instrument-free digital measurements: a three-dimensional microfluidic device fabricated in a single sheet of paper by double-sided printing and lamination
Toward instrument-free digital measurements: a three-dimensional microfluidic device fabricated in a single sheet of paper by double-sided printing and lamination
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DOI：
10.1039/c4lc01382d
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发表时间：
2015-01-01
期刊：
影响因子：
6.1
通讯作者：
Lee, Chang-Soo
中科院分区：
文献类型：
--
作者：
Jeong, Seong-Geun;Lee, Sang-Ho;Lee, Chang-Soo
这项研究展示了一种简单的方法，从一张纸双面印刷和层压制造的3D μ PAD。首先，蜡打印机打印垂直对称和不对称的蜡图案到双面纸表面。然后，层压机熔化印刷的蜡图案以在纸张中形成微流体通道。当熔化的蜡模彼此接触时，垂直对称的蜡模形成垂直通道。当印刷的蜡模熔化到比单张纸的厚度低的高度时，不对称的蜡模在纸的横截面处形成横向和垂直通道。最后，两种类型的蜡模形成3D微流体网络，以在单张纸中横向和垂直移动流体。这种方法消除了与复杂和繁琐的对准、组装、粘合和冲压过程相关的主要技术障碍。该3D-mu PAD可用于多重数字测定，通过在固定时间计数彩色条的数量来简单地测量样品溶液中目标分析物的浓度。它不需要任何外部仪器来执行数字测量。因此，我们希望这种方法可以成为一种在发展中国家使用的无仪器测定形式。
This study demonstrates a simple approach for fabricating a 3D-mu PAD from a single sheet of paper by double-sided printing and lamination. First, a wax printer prints vertically symmetrical and asymmetrical wax patterns onto a double-sided paper surface. Then, a laminator melts the printed wax patterns to form microfluidic channels in the paper sheet. The vertically symmetrical wax patterns form vertical channels when the melted wax patterns make contact with each other. The asymmetrical wax patterns form lateral and vertical channels at the cross section of the paper when the printed wax patterns are melted to a lower height than the thickness of the single sheet of paper. Finally, the two types of wax patterns form a 3D microfluidic network to move fluid laterally and vertically in the single sheet of paper. This method eliminates major technical hurdles related to the complicated and tedious alignment, assembly, bonding, and punching process. This 3D-mu PAD can be used in a multiplex digital assay to measure the concentration of a target analyte in a sample solution simply by counting the number of colored bars at a fixed time. It does not require any external instruments to perform digital measurements. Therefore, we expect that this approach could be an instrument-free assay format for use in developing countries.