Protective effects of Chinese propolis and its component, chrysin, against neuronal cell death via inhibition of mitochondrial apoptosis pathway in SH-SY5Y cells
Protective effects of Chinese propolis and its component, chrysin, against neuronal cell death via inhibition of mitochondrial apoptosis pathway in SH-SY5Y cells
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DOI：
10.1021/jf8014206
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发表时间：
2008-10-08
影响因子：
6.1
通讯作者：
Hara, Hideaki
中科院分区：
文献类型：
--
作者：
Izuta, Hiroshi;Shimazawa, Masamitsu;Hara, Hideaki
内质网（ER）应激与神经退行性疾病和缺血性疾病的发病机制有关。本研究的目的是评价中国蜂胶及其成分[菊花素、高良姜素、皮诺曹蛋白、咖啡酸和咖啡酸苯乙酯（CAPE）]对tunicamycin诱导的SH-SY5Y细胞死亡的影响。中国蜂胶和菊花素浓度依赖性地抑制了这种细胞死亡、tunicamycin诱导的caspase-3激活以及tunicamycin对线粒体的影响[细胞色素c释放到细胞质中并破坏线粒体膜电位（Delta Psi(m)）]。此外，蜂胶和菊花素均能抑制星孢素诱导的细胞死亡。这些结果表明，蜂胶对内质网应激或星孢素诱导的神经元细胞死亡的抑制作用可能主要是由菊花素发挥的。此外，保护作用的机制可能至少部分涉及抑制caspase-3活性和线粒体凋亡途径。
Endoplasmic reticulum (ER) stress has been implicated in the pathogenesis of neurodegenerative and ischemic disorders. The purpose of this study was to evaluate the effects of Chinese propolis and its constituents [chrysin, galangin, pinocembrin, caffeic acid, and caffeic acid phenethyl ester (CAPE)] against tunicamycin-induced neuronal cell death in SH-SY5Y cells. Both Chinese propolis and chrysin concentration-dependently inhibited such cell death, the tunicamycin-induced activation of caspase-3, and the effects of tunicamycin on mitochondria [release of cytochrome c into the cytosol and disruption of the mitochondrial membrane potential (Delta Psi(m))]. Furthermore, Chinese propolis and chrysin each inhibited staurosporine-induced cell death. These findings indicate that the inhibitory effects of Chinese propolis against neuronal cell death induced by ER stress or staurosporine may be exerted primarily by chrysin. Moreover, the mechanism underlying the protective effects may, at least partly, involve inhibitions of caspase-3 activity and the mitochondrial apoptotic pathway.