EfaR Is a Major Regulator of Enterococcus faecalis Manganese Transporters and Influences Processes Involved in Host Colonization and Infection
EfaR Is a Major Regulator of Enterococcus faecalis Manganese Transporters and Influences Processes Involved in Host Colonization and Infection
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DOI：
10.1128/iai.06377-11
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发表时间：
2013-03-01
影响因子：
3.1
通讯作者：
Lopes, M. de F.
中科院分区：
文献类型：
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作者：
Abrantes, M. C.;Kok, J.;Lopes, M. de F.
金属离子，特别是锰，是细菌致病性的重要调节剂。然而，锰依赖蛋白在医院病原体粪肠球菌中的作用知之甚少，粪肠球菌是细菌性心内膜炎的主要原因。本研究表明，粪肠杆菌的DtxR/MntR家族金属调节因子EfaR以锰依赖的方式控制其几个调控成员的表达。我们还表明，efaR失活会损害粪肠球菌形成生物膜、在巨噬细胞内存活和耐受氧化应激的能力。我们的研究结果表明，EfaR是粪肠球菌毒力的重要调节剂，并将锰稳态与肠球菌致病性联系起来。
Metal ions, in particular manganese, are important modulators of bacterial pathogenicity. However, little is known about the role of manganese-dependent proteins in the nosocomial pathogen Enterococcus faecalis, a major cause of bacterial endocarditis. The present study demonstrates that the DtxR/MntR family metalloregulator EfaR of E. faecalis controls the expression of several of its regulon members in a manganese-dependent way. We also show that efaR inactivation impairs the ability of E. faecalis to form biofilms, to survive inside macrophages, and to tolerate oxidative stress. Our results reveal that EfaR is an important modulator of E. faecalis virulence and link manganese homeostasis to enterococcal pathogenicity.