Adverse effect of tannery waste leachates in transgenic Drosophila melanogaster: role of ROS in modulation of Hsp70, oxidative stress and apoptosis
Adverse effect of tannery waste leachates in transgenic Drosophila melanogaster: role of ROS in modulation of Hsp70, oxidative stress and apoptosis
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DOI：
10.1002/jat.1332
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发表时间：
2008-08
影响因子：
3.3
通讯作者：
Hifzur R Siddique;Subash C. Gupta;K. Mitra;V. K. Bajpai;N. Mathur;R. Murthy;D. K. Saxena;D. K. Chowdhuri
中科院分区：
文献类型：
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作者：
Hifzur R Siddique;Subash C. Gupta;K. Mitra;V. K. Bajpai;N. Mathur;R. Murthy;D. K. Saxena;D. K. Chowdhuri
渗滤液是一种复杂的化学混合物，由固体废物的沥滤产生。渗滤液的潜在毒性是一个主要的环境健康问题。本研究评价了活性氧在制革浸出液诱导果蝇Hsp 70表达、抗氧化酶和细胞凋亡中的作用。将不同浓度（0.05-2.0%）的制革废物在不同pH（7.00、4.93和2.88）下制备的沥滤液与果蝇食物混合，并喂食幼虫2-48 h，以检查不同的应激和凋亡标记。在暴露的幼虫中观察到Hsp 70表达、ROS产生、抗氧化酶活性和MDA含量的浓度和时间依赖性显著增加。抗氧化酶的活性与Hsp 70表达和MDA水平相比，在暴露的生物体中被延迟。在较高浓度下暴露的幼虫中观察到细胞凋亡，同时Hsp 70沿着显著消退，并产生较高水平的ROS。在这些浓度下，ROS生成与凋亡标记物之间的正相关性和凋亡标记物与Hsp 70表达之间的负相关性表明ROS在暴露生物体中诱导细胞损伤中的重要作用。暴露于0.5%浸出物的幼虫中产生了大量的活性氧，但这并不干扰Hsp 70和抗氧化酶对其细胞的保护。然而，在暴露于1.0%和2.0%沥滤物的幼虫中产生显著更高水平的ROS可能会降低Hsp 70表达，从而导致线粒体介导的caspase依赖性凋亡细胞死亡。版权所有© 2008约翰威利父子有限公司.
Leachate is a complex chemical mixture of chemicals produced as a result of leaching of solid wastes. The potential toxicity of leachates is a major environmental health concern. The present study evaluated the role of ROS in tannery leachates induced Hsp70 expression, antioxidant enzymes and apoptosis in Drosophila. Different concentrations (0.05–2.0%) of leachates prepared from tannery waste at different pH (7.00, 4.93 and 2.88) were mixed with Drosophila food and fed to the larvae for 2–48 h to examine the different stress and apoptotic markers. A concentration‐ and time‐dependent significant increase in Hsp70 expression, ROS generation, antioxidant enzymes activities and MDA content were observed in the exposed larvae. Activities of antioxidant enzymes were delayed compared with Hsp70 expression and MDA level in the exposed organisms. Apoptotic cell death was observed in the exposed larvae at higher concentrations concurrent with a significant regression in Hsp70 along with a higher level of ROS generation. A positive correlation drawn between ROS generation and apoptotic markers and a negative correlation between apoptotic markers and Hsp70 expression at these concentrations indicated the important role of ROS in the induction of cellular damage in the exposed organisms. There was a significant generation of ROS in the larvae exposed to 0.5% of leachates which did not interfere with the protection of their cells by Hsp70 and antioxidant enzymes. However, generation of significantly higher levels of ROS in the larvae exposed to 1.0% and 2.0% leachates may decrease Hsp70 expression thus leading to mitochondria‐mediated caspase‐dependent apoptotic cell death. Copyright © 2008 John Wiley & Sons, Ltd.