Rickettsia slovaca and Rickettsia raoultii in Dermacentor marginatus and Dermacentor reticulatus ticks from Slovak Republic
Rickettsia slovaca and Rickettsia raoultii in Dermacentor marginatus and Dermacentor reticulatus ticks from Slovak Republic
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DOI：
10.1007/s10493-012-9539-8
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发表时间：
2012-06-01
影响因子：
2.2
通讯作者：
Boldis, Vojtech
中科院分区：
文献类型：
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作者：
Spitalska, Eva;Stefanidesova, Katarina;Boldis, Vojtech
立克次体是专性细胞内革兰氏阴性细菌，与节肢动物媒介有关，可导致人类轻度至重度疾病。是已知的传播媒介并分布在欧洲各地。在斯洛伐克，从马、绵羊、山羊和狗身上采集了总共 794 只蜱虫和成年蜱虫。 sp的DNA。通过 PCR 扩增 、 和 基因部分，在 229 只蜱中发现了 。接下来通过 PCR-RFLP 和/或测序对 阳性样本进行的分析表明，蜱虫感染的程度更高，并且感染的程度更高。和 的患病率分别为 8.1-8.6% 和 22.3-27%。患病率为 20.6-24.3%，而 1.7-3.4%。通过基于 rOmpA 的定量 SybrGreen PCR 测定评估蜱分离株的细胞内生长。 Vero 和 L929 细胞分别在感染后第 7 天和第 8 天记录了增殖的最高点。在给豚鼠喂食阳性蜱期间传播立克次体，随后在所有器官中都记录到立克次体感染，感染最高的是脾、肝和心脏。我们的研究描述了蜱传病原体的检测和分离，表明它们在斯洛伐克传播，并强调了它们对人类的风险。
Rickettsiae, obligate intracellular Gram-negative bacteria, responsible for mild to severe diseases in humans are associated with arthropod vectors. and are known vectors of and distributed across Europe. A total of 794 , and adult ticks were collected from the vegetation, removed from horses, sheep, goats and dogs in Slovakia. The DNA of sp. was found in 229 ticks by PCR amplifying parts of , and genes. Next analyses of -positive samples by PCR-RFLP and/or sequencing showed ticks were more infected with and were more infected with . The prevalence of was 8.1-8.6% and 22.3-27% in and , respectively. The prevalence of was 20.6-24.3% in and 1.7-3.4% in . Intracellular growth of isolate from tick was evaluated by rOmpA-based quantitative SybrGreen PCR assay. The highest point of multiplication was recorded on the 7th and 8th day postinfection in Vero and L929 cells, respectively. was transmitted during feeding of -positive ticks to guinea pigs and subsequently rickettsial infection was recorded in all organs, the highest infection was in spleen, liver and heart. Our study describes the detection and isolation of tick-borne pathogens and , show that they are spread in Slovakia and highlight their risk for humans.