Angiogenesis and tumor progression of melanoma. Quantification of vascularity in melanocytic nevi and cutaneous malignant melanoma.
Angiogenesis and tumor progression of melanoma. Quantification of vascularity in melanocytic nevi and cutaneous malignant melanoma.
复制标题
黑色素瘤的血管生成和肿瘤进展。
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
B. Hyman
中科院分区：
文献类型：
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作者：
R. Barnhill;K. Fandrey;Levy Ma;M. Mihm;B. Hyman
背景
皮肤恶性黑色素瘤(CMM)诱导血管生成的能力是公认的。此外，据报道，与常见的获得性痣相比，发育不良的黑色素细胞痣(DMN)表现出明显的血管丰富；但这一观察结果从未得到客观证实。
实验设计
在接下来的研究中，对10例正常皮肤、18例黑色素细胞痣、29例DMN、37例原发CMM和5例黑色素瘤转移瘤的乳头状真皮或肿瘤血管进行了定量。用凝集素Ulex uropaeus凝集素I鉴定微血管，用一个眼格(面积7.84×10(-2)mm2)在x400倍率下计数微血管的数量，并记录每个标本5个视野的平均血管数。
结果
平均血管计数：正常皮肤5.9条，普通获得性痣9.1条，表现为DMN 10.3条，DMN，轻度不典型11.8条；DMN，中度不典型12.2条；DMN，重度不典型14.8条；原发CMM 25.4条；转移性黑色素瘤29.5条)。DMN、重度非典型性痣与黑色素瘤(P<0.01)、DMN、重度痣与普通痣(P<0.02)以及具有DMN特征的痣(P<0.05)之间的差异有统计学意义。径向生长期CMM与垂直生长期CMM、小于1.0 mm的CMM与大于1.0 mm的CMM的微血管计数比较，差异均无统计学意义。然而，放射状生长的CMM与严重不典型的DMN确实不同(22.4vs14.8，p<0.05)。
结论
这些结果首次量化了在CMM的放射状生长阶段，随着黑素细胞系统中肿瘤的进展和血管生成的开始，血管逐渐增加。除严重不典型DMN外，DMN在整体血管方面与普通型色素痣无显著差异。
BACKGROUND
The capacity of cutaneous malignant melanoma (CMM) to induce angiogenesis is well established. In addition, dysplastic melanocytic nevi (DMN) have been reported to display prominent vascularity relative to common acquired nevi; but this observation has never been verified objectively.
EXPERIMENTAL DESIGN
In the following studies, papillary dermal or tumor vascularity was quantified in 10 examples of normal skin, and in a series of 18 melanocytic nevi, 29 DMN, 37 primary CMM and 5 melanoma metastases. Microvessels were identified with the lectin Ulex europaeus agglutinin I. The number of microvessels were counted with an ocular grid (area 7.84 x 10(-2) mm2) at x400 magnification, and the mean vascularity recorded for five fields for each specimen.
RESULTS
Mean vascular counts were as follows: normal skin 5.9, common acquired nevus 9.1, nevus with features of DMN 10.3, DMN, slight atypia 11.8; DMN, moderate atypia 12.2; DMN, severe atypia 14.8; primary CMM 25.4; and metastatic melanoma 29.5). Significant differences were recorded for DMN, severe atypia versus melanoma (p less than 0.01), DMN, severe versus common nevi (p less than 0.02) and versus nevi with features of DMN (P less than 0.05). When microvessel counts from CMM in the radial growth phase were compared with those from CMM in the vertical growth phase, or CMM less than 1.0 mm versus those greater than 1.0 mm, no significant differences were found. However, CMM in radial growth did differ from severely atypical DMN (22.4 versus 14.8, p less than 0.05).
CONCLUSIONS
These results quantify for the first time a gradual rise in vascularity with tumor progression in the melanocytic system and onset of angiogenesis during the radial growth phase of CMM. Other than severely atypical DMN, DMN did not differ substantially from common nevi with reference to overall vascularity.