DIFFERENTIAL BINDING OF ALKYLATING FLUOROCHROMES IN HUMAN CHROMOSOMES
DIFFERENTIAL BINDING OF ALKYLATING FLUOROCHROMES IN HUMAN CHROMOSOMES
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DOI：
10.1016/0014-4827(70)90523-9
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发表时间：
1970-01-01
影响因子：
3.7
通讯作者：
JOHANSSO.C
中科院分区：
文献类型：
--
作者：
CASPERSS.T;ZECH, L;JOHANSSO.C
用奎纳克林芥子气（QM）处理血培养物中的人中期染色体，显示荧光条带图案，在具有最强荧光区域（3，13-15和Y）的染色体中，发现该荧光条带图案是恒定的和可重复的。正如在植物材料中研究的早期，这种模式出现的特点，它可以帮助染色体鉴定。改进的荧光技术也可以对较小的人类染色体进行详细的研究。在QM结合的强能力与冷处理（根据达林顿和La Cour）或胸苷标记技术所确定的异染色质分布之间似乎存在某种相关性。
Human metaphase chromosomes from blood cultures, treated with quinacrine mustard (QM), show a banded pattern of fluorescence, which in the chromosomes with the most strongly fluorescent regions (3, 13–15 and Y) was found to be constant and reproducible. As in plant materials studied earlier, this pattern appears so characteristic that it can be of help for chromosome identification. Improved fluorescence techniques should permit detailed studies on smaller human chromosomes as well.A certain correlation appears to exist between strong capacity for QM-binding and the distribution of heterochromatin as defined by cold treatment (according to Darlington & La Cour) or thymidine labelling-techniques.